新型生物材料聚丁二酸丁二醇酯体外水解性能  

Degradation of poly(butylenes succinate) as a novel biomaterial in vitro

作　　者：石峰晖[1,2] 王晓青[3] 季君晖[1] 王进锋[1] 刘伟[1] 严庆[1] 

机构地区：[1]中国科学院理化技术研究所 [2]中国科学院研究生院,北京市100049 [3]北京理工大学

出　　处：《中国组织工程研究与临床康复》2008年第23期4473-4476,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景：聚丁二酸丁二醇酯类聚酯是一种新型脂肪族聚酯，因其具有良好的生物降解性、优异的力学性能和成型加工性而倍受青睐，是一种潜在的组织工程支架材料和缓释药物载体材料。目的：验证新型可降解脂肪族聚酯聚丁二酸丁二醇酯的体外降解行为。设计、时间及地点：单一样本观察，于2007—06／11在中科院理化所工程塑料国家工程研究中一心完成。材料：聚丁二酸丁二醇酯由丁二酸和丁二醇通过熔融缩聚制备，粗产品用氯仿溶解后乙醇沉淀提纯，60℃真空干燥48h后备用。方法：用平板硫化机将样品压制成100um薄膜，然后裁成lcm×lcm的小片。在pH值为7．4的磷酸盐缓冲液中进行体外水解实验。主要观察指标：分析聚丁二酸丁二醇酯的质量、分子量、pH值和表面形态的变化。结果：①聚丁二酸丁二醇酯薄膜在磷酸缓冲溶液中发生降解，降解基本分为两个阶段。0-9周为降解停滞阶段，质量基本保持不变，9～15周加速降解阶段，15周的质量保持率为46％。降解实验中分子量的直线下降，没有停滞阶段。②降解过程中产生的酸性物质导致缓冲溶液pH值有一定的升高，介于6．0～8.0之间。③聚丁二酸丁二醇酯薄膜表面首先变得粗糙，出现很多小裂纹。降解12周后，样品的表面均出现裂缝，随着降解时间的裂缝的数量增加、缝隙变大，降解15周后，样品的裂缝进一步加大，变多，与典型的本体降解特征一致。结论：聚丁二酸丁二醇酯在模拟体液的磷酸缓冲溶液中可以发生降解，降解行为分为停滞阶段和加速降解阶段，降解中产生酸性物质。BACKGROUND： Poly（butylene succinate） （PBS） has also been known as an important biodegradable aliphatic polyester because of its good degradation properties, excellent physical properties and molding processability, Therefore, its potential application as a novel biomaterial has been concerned and expected to be used as the drug carriers in controlled delivery and the scaffold materials in tissue engineering. OBJECTIVE： To verify the degradation behavior of the biodegradable PBS in vitro. DESIGN, TIME AND SETTING： The experiment, an unitary sample observation, was conducted in the State Engineering Research Center of Engineering Plastics, Institute of Physics and Chemistry, Chinese Academy of Sciences （Beijing, China） from June to November in 2007. MATERIALS： PBS was synthesized by melt phase polycondensation of 1,4-butanediol and succinic acid, then purified in the sequence of being dissolved in chloroform, precipitated in methanol, and dried in a vacuum at 60 ℃for 48 hours. METHODS： The average thickness of 100 μ m PBS film was prepared by heat-processing method using plate vulcanization machine, and then cut into pieces of 1 cm× 1 cm area. The evaluation of PBS film degradation behavior was carried out in the phosphate-buffered saline （pH 7.4）. MAIN OUTCOME MEASURES： The changes in weight, molecular weight, pH value and surface structures of PBS were examined. RESULTS： PBS films degraded in the phosphate-buffered saline solution. The degradation process exhibited two phases： The degradation in the first phase was slow in 0-9 weeks, and the process accelerated in the second phase of 9-15 weeks. Unlike the weight change, the molecular weight of the PBS films decreased immediately and continually throughout the time course. The pH value of the phosphate-buffered saline solution remained between 6.0 and 8.0 though an increase appreciably after degradation, which resulted from the acid degradation products of the polymer. The PBS films became rough and a large number of

关 键 词：生物材料 聚丁二酸丁二醇酯 降解 

分 类 号：R318[医药卫生—生物医学工程]