简单快速制备壳聚糖支架导管的方法  

Preparation of chitosan scaffold tube

作　　者：陈刚[1] 石文君[1] 

机构地区：[1]中国医科大学第二临床学院胸外科,辽宁省沈阳市110003

出　　处：《中国组织工程研究与临床康复》2008年第23期4477-4480,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：卫生部立项资助课题;课题名称:肺组织瓣重建气管食管的应用研究。(WKZ-2000-1-17)~~

摘　　要：背景:乳液冷冻干燥法是近年来最常用制备壳聚糖的方法,制备工艺复杂。目的:介绍一种简单的壳聚糖支架导管制备方法并分析其生物特性。设计、时间及地点:对比观察实验,于2007-04/12在中国医科大学附属第二医院中心实验室完成。材料:SPF级出生8周的Wistar大鼠10只。散装医用级壳聚糖粉,纯度99%,脱乙酰度为99%,为山东济南海洋生物制品有限公司产品。方法:利用乙酸溶液制备8%壳聚糖乙酸水溶胶,溶胶放置1d,将硬塑料管浸入溶胶后迅速取出放入1mol/L氢氧化钠液内3min,见管外溶胶变白凝固成胶冻状,取出后晾到五分干后轻轻退下内管,晾干后即成为壳聚糖导管。取内径5mm壳聚糖管,切成段,长度2mm,将其植入大鼠右侧麦氏点皮下肌肉组织内,同时在大鼠左侧相当于麦氏点处切口,分离到肌肉层,不植入壳聚糖管,单纯缝合,作为对照。主要观察指标:壳聚糖管的大体观察,壳聚糖管在大鼠肌肉组织中的降解百分比,苏木精-伊红染色观察壳聚糖管周组织炎症变化。结果:制成的壳聚糖导管光滑、坚韧。肌内埋植实验显示,术后4,8周壳聚糖管可分别被组织吸收50%和90%以上,管周组织炎性反应逐渐减退。结论:采用该方法制备壳聚糖管具有所需材料少,成本低廉,制作方法简单,制作时间短的特点,并证实所制备壳聚糖管具有良好的组织相容性和生物可降解性。BACKGROUND： Chitosan is commonly prepared using emulsion freeze-drying methods, which is complexly operated. OBJECTIVE： To introduce a simple methods for the preparation of chitosan and analyze its biological characteristic. DESIGN, TIME AND SETTING： Controlled observation experiments were carried out in the Central Laboratory, the Second Affiliated Hospital of Chinese Medical University （Shenyang, Liaoning, China） from April to December in 2007. MATERIALS： Ten Wistar rats of 8 weeks old and SPF grade were adopted. Chitosan powder in separated packs, with 99% purity and 99% deacetylation degree were the product of Shandong Jinan Ocean Bio-Product Co., Ltd. （China）. METHODS： Acetic acid solution was added into chitosan to prepare 8% chitosan acetic acid hydrosol, which was then stayed for one day. Flowing the immersion into the hydrosol and immediate dislodgement, plastic tube was soaked in 1 mol/L sodium hydroxide for 3 minutes. Hydrosol out of the tube etiolated and coagulated into jells, the tube was removed when the jells were semi-dried. Subsequent to complete drying, chitosan tubes were prepared as an internal diameter of 5 mm, and were cut into many segmentations （length 2 mm）. The prepared tubes were subcutaneously implanted into rat muscular tissues at right McBurney point, while rat left muscular tissues were only incised and sutured, taking as controls. MAIN OUTCOME MEASURES： Gross observation of chitosan tube, degradation percentage of chitosan tubes in rat muscular tissue, and tissue inflammation change around chitosan tube detected by hematoxylin-eosin stain. RESULTS： The surface of chitosan tube was smooth and the tenacity was good. Intramuscular implantation test showed that 50% and 90% chitosan tubes were absorbed by tissues in 4 weeks and 8 weeks postoperatively. Inflammatory reaction surrounding tubes was decreased gradually. CONCLUSION： It gives a new kind of way to prepare chitosan tube, with few materials, low cost, simple operation and short time. Th

关 键 词：壳聚糖 生物高分子材料 导管支架 生物降解 

分 类 号：R318[医药卫生—生物医学工程]