抗中性粒细胞胞质抗体对中性粒细胞表面FcγⅡ／Ⅲ受体表达的影响及其意义  

The expression of neutrophils FcγⅡ/Ⅲ modulated by anti-neutrophil cytoplasmic antibodies and its clinical value

作　　者：王湘玲[1] 陈楠[1] 俞海瑾[1] 任红[1] 王伟铭[1] 倪莉燕[1] 

机构地区：[1]上海交通大学附属瑞金医院肾脏科,200025

出　　处：《中华风湿病学杂志》2008年第7期442-444,I0001,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目（C030204）;上海市重点学科资助项目（T0201）;上海市卫生局重点学科资助项目（05111001）;上海高校选拔培养优秀青年教师科研专项基金资助项目（18032）

摘　　要：目的探讨抗中性粒细胞胞质抗体（ANCA）对中性粒细胞表面FcγⅡ／Ⅲ（CD32／CD16）受体表达的影响及临床意义。方法提纯活动期ANCA相关性小血管炎（AASV）患者的ANCAIgG，分离健康人新鲜中性粒细胞，以ANCA刺激粒细胞1h后，流式细胞仪检测CD32／CD16的表达。流式细胞仪检测18例系统性小血管炎患者CD32／CD16的表达，以35名健康人为对照。同时对18例患者临床表现及血管炎活动情况进行评分，与CD32／CD16表达进行相关分析。结果ANCAIgG与正常人IgG相比显著上调中性粒细胞表面CD16的表达（Mnx67±23vss54±21，P〈0．01），但对CD32的表达无影响（Mnx21±8vs16±8，P〉0．05）。系统性小血管炎患者CD16表达的平均荧光强度显著高于正常人（Mnx62±12vs53±10，P〈0．01），而CD32的表达与正常人差异无统计学意义。CD16表达与伯明翰小血管炎呈显著正相关（P〈0．01）。结论ANCA相关性小血管炎患者高表达Fcγ受体，ANCA可以干预CD32／CD16受体的表达，调控Fcγ受体表达可能是系统性小血管炎的发病机制之一，监测CD16的表达水平对于判断疾病活动具参考价值。Objective The interaction between anti-neutrophil cytoplasmic antibaties （ANCA） and receptors at the Fc portion of immunoglobulins （ FcγR ） is central in the pathogenisis of primary systemic small vasculitis. The aim of this study is to investigate the role and clinical value of ANCA on the expression of neutrophils FcγⅡ/Ⅲ （CD32/CD16）. Methods ANCA IgG was prepared from the sera of patients with active We-gener＇s granulomatosis （WG） and microscopic polyangiitis （ MPA ）. Neutrophils were isolated from the blood of healthy volunteers. The expression of CD32/CD16 on neutrophils was assessed by flow cytometry after stimulated by ANCA for 1 hour. We compared the expression of CD32/CD16 between 18 primary systemic small vasculitis （PSV） patients and 35 healthy volunteers. Furthermore, the correlation was also be analyzed between the expression of CD32/CD16 and Birmingham vasculitis activity score （BVAS）. Results The expression of CD16 was significantly elevated by ANCA （Mnx 67±23 vs 54±21, P〈0.01）. The expression of CD16 was higher in patients than in healthy volunteers （Mnx 62±12 vs 53±10, P〈0.01）, which was in correlation with BVAS （r=0.728 86, P〈0.01 ）. But no such eor,＇elation was found for CD32 . Conclusion ANCA may play a role in the pathogenesis of PSV by modulating the expression of the FCγR. Monitoring the expression of CD16 on neutrophils is helpful for the evaluation of PSV activity.

关 键 词：抗体 抗中性粒白细胞胞质 受体 IgG 血管炎 

分 类 号：R593.2[医药卫生—内科学]