Analysis of anti-platelet aggregation components of Rhizoma Zingiberis using chicken thrombocyte extract and high performance liquid chromatography  

作　　者：NIE Hong MENG Lan-zhen ZHANG Hui ZHANG Jian-yu YIN Zhen HUANG Xue-song 

机构地区：[1]Jinan University College of Pharmacy, Guangzhou, Guangdong 510632, China [2]Xinxiang Medical College, Xinxiang, Henan 453003, China [3]Jinan University College of Science and Engineering, Guangzhou, Guangdong 510632, China

出　　处：《Chinese Medical Journal》2008年第13期1226-1229,共4页中华医学杂志（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （No. 30400596）, Jinan University Natural Science Foundation （No. 51204017） and the Chinese Traditional Medicine Foundation of Guangdong Province （No. 104005）.

摘　　要：Background The conventional procedure for screening bioactive components from traditional Chinese medicine is time-consuming, expensive and low efficient. Therefore, some alternative strategies are needed urgently. A novel method for screening anti-platelet aggregation components from oleoresins was developed using chicken thrombocyte extract and high performance liquid chromatography. Methods The anti-platelet aggregation components of oleoresins were combined with receptors, channels and enzymes of chicken thrombocytes under physiological environment. Unbound substances were washed away and bound compounds were eluted using specific phosphate buffered solution （PBS）. Compounds released from target sites were collected and analyzed by high performance liquid chromatography and LC-MS. The activity of three compounds which were screened from this model was confirmed using platelet aggregation pharmacology in vivo. Results There were four typical compounds that bound to the thrombocytes： 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol, and all had shown anti-platelet aggregation activities. Eight-gingerol displayed the best anti-platelet aggregation effect. Conclusions Chicken thromobcyte extract can be used to isolate chemicals that are ligands of the receptor or other bio-targets on the platelet. This may therefore be a simple and efficient method to screen for anti-platelet aggregation compounds from traditional Chinese medicine.Background The conventional procedure for screening bioactive components from traditional Chinese medicine is time-consuming, expensive and low efficient. Therefore, some alternative strategies are needed urgently. A novel method for screening anti-platelet aggregation components from oleoresins was developed using chicken thrombocyte extract and high performance liquid chromatography. Methods The anti-platelet aggregation components of oleoresins were combined with receptors, channels and enzymes of chicken thrombocytes under physiological environment. Unbound substances were washed away and bound compounds were eluted using specific phosphate buffered solution （PBS）. Compounds released from target sites were collected and analyzed by high performance liquid chromatography and LC-MS. The activity of three compounds which were screened from this model was confirmed using platelet aggregation pharmacology in vivo. Results There were four typical compounds that bound to the thrombocytes： 6-gingerol, 8-gingerol, 6-shogaol and 10-gingerol, and all had shown anti-platelet aggregation activities. Eight-gingerol displayed the best anti-platelet aggregation effect. Conclusions Chicken thromobcyte extract can be used to isolate chemicals that are ligands of the receptor or other bio-targets on the platelet. This may therefore be a simple and efficient method to screen for anti-platelet aggregation compounds from traditional Chinese medicine.

关 键 词：Rhizoma Zingiberis chicken thrombocytes GINGEROL platelet aggregation high performance liquid chromatography 

分 类 号：R285[医药卫生—中药学]