羊驼酪氨酸酶基因家族在不同毛色个体中的基因表达水平  

Gene Expression Levels of Alpaca Tyrosinase Gene Family in Individuals of Different Colors

作　　者：高莉[1] 董常生[1] 赫晓燕[1] 贺俊平[1] 耿建军[1] 范瑞文[1] 朱芷薇[1] 游蓉丽[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2008年第7期895-899,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30501070)

摘　　要：黑色素对动物毛色的形成起重要作用,酪氨酸酶基因家族成员参与了黑色素的合成。为了探索羊驼酪氨酸酶基因家族的基因表达与其毛色之间的相关性,本研究利用实时荧光定量PCR技术分析了不同毛色羊驼酪氨酸酶基因家族成员TYR、TRP1和TRP2的相对基因表达量。研究结果显示:棕色羊驼中的TYR、TRP1和TRP2的mRNA相对表达量经内参基因校正后分别是白色羊驼中的13.669倍、3.417倍和8.593倍。结论表明棕色羊驼中酪氨酸酶基因家族3种成员的基因表达水平均高于白色羊驼,揭示了羊驼TYR基因家族成员的基因表达量与其毛色表型具有相关性。Melanin plays important roles in the formation of animal hair color, the members of TYR gene family participate in the synthesis of melanin. For exploring the relationship between gene expression of alpaca TYR gene family and alpaca＇s coat color, the relative expression quantity of TYR, TRP1, TRP2 in alpaca of different colors were analyzed by using real-time quantitative PCR in this research. Results showed that the relative expression quantity of TYR,TRP1, TRP2 in Brown alpaca respectively were 13. 669,3. 417,8. 593 times than that in White alpaca, all results were corrected by the household gene. The findings indicated that, gene expression level of TYR gene family in Brown alpaca were higher than that in White alpaca, and the gene expression level of TYR gene family were related with phenotype of alpacas＇ coat color.

关 键 词：羊驼 TYR TRP1 TRP2 实时荧光定量PCR 

分 类 号：S511[农业科学—作物学] Q75[生物学—分子生物学]