商品蛋鸡成髓细胞瘤、血管瘤型J亚群白血病病理学初报  

Preliminary Pathology Report of Myeloblastosis and Hemangioma Associated with ALV-J in Commercial Layer Chicken

作　　者：成子强[1] 刘思当[1] 孟祥凯[1] 张洪海[1] 刁秀国[1] 刘青[1] 崔治中[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《畜牧兽医学报》2008年第7期935-940,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省科技攻关项目(2006GG2209010);国家博士后基金及二等奖励金资助项目(2005038258)

摘　　要：2007年7月,山东某鸡场8 000余只150日龄海兰褐鸡发病,病鸡特征性表现衰竭、消瘦和血管瘤。大体病变:内脏器官肿大,表面及切面可见大小不等的灰白色结节;血管瘤大小不等、富有弹性,内含大量血液,血液内富含成髓细胞;组织学病变:肝脏、脾脏、骨髓、肠管和胰腺浆膜面有大量的胞质含嗜碱性颗粒的成髓细胞增生,同时在肠管固有层出现明显的肠腺上皮细胞和网状细胞增生,腺胃固有层出现明显的网状细胞增生,血管瘤为典型的海绵状血管瘤,可见其发展的各个阶段;免疫组织化学检测:经REV与ALV-J单克隆抗体检测发现,REV抗原阴性,而ALV-J抗原强阳性。在骨髓、肝脏及其它组织内的成髓细胞内、血管瘤海绵状壁层内皮细胞、增生的肠腺上皮细胞、腺胃腺小管上皮细胞、网状细胞内及部分间质内可见强阳性信号。因此,此病为成髓细胞瘤、血管瘤型并伴发多种肿瘤的J亚群白血病,这在国内外尚未见报道。In July 2007, myeloblastosis and hemangioma cases associated with avian leukosis virus subgroup J （ALV-J） were first diagnosed in a layer chicken（150-day） flock in Shandong province. The main symptoms were characterized by runting-stunting and hemangioma. In the necropsy, almost all viscera tissues showed moderate to severe enlargement with diffuse gray-white nodules; Cutting open the hemangioma, blood effused and many myeloblasts were seen in blood smear. Histological examination indicated that the tumor cells proliferated in tissues mainly were myeloblasts with basophilia granules in cytoplasm; Intestinal gland epithelial cells and reticulocytes proliferated in lamina propria of alimentary tract; Hemangioma were typical sponge-like hemangioma. By immunohistochemistry （IHC） technique with ALV-J and REV monoclonal antibodies, the most intense staining of ALV-J antigen was found in the tumor tissues, liver, spleen, bone marrow, and proventriculus, while REV antigen was negative. The results indicated that myeloblastosis, hemangioma and other tissues tumors occurred in layer chickens were new dominance tumors induced by avian leukosis virus subgroup J （ALV-J）.

关 键 词：商品蛋鸡 成髓细胞瘤 血管瘤 J亚群白血病 病理学 免疫组织化学 

分 类 号：S858.315.3[农业科学—临床兽医学]