猪带绦虫六钩蚴TSOL18基因疫苗表达载体的构建及其免疫原性研究  

Construction of Gene Vaccine Vector Carrying TSOL18 Gene of Taenia solium Oncosphere and Its Immunogenicity

作　　者：郭爱疆[1] 才学鹏[2] 房永祥[2] 贾万忠[2] 骆学农[2] 刘红霞[2] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,兰州730046

出　　处：《畜牧兽医学报》2008年第7期945-949,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家高技术研究发展计划“863”项目(2003AA241111)

摘　　要：为研制新型猪囊尾蚴疫苗,将猪带绦虫六钩蚴阶段编码TSOL18的基因定向克隆于真核表达质粒pVAX1,经筛选、鉴定及DNA序列分析正确后,将重组质粒pVAX1/TSOL18转染BHK-21细胞,通过SDS-PAGE、Western blotting、免疫荧光试验等方法检测细胞中表达的TSOL18抗原。结果表明,重组真核质粒pVAX1/TSOL18可在BHK-21细胞中表达TSOL18目的蛋白,表达蛋白能被猪囊尾蚴病阳性血清所识别。动物免疫试验表明,真核表达载体能有效地诱导机体产生细胞免疫和体液免疫应答,这为猪囊尾蚴病基因疫苗的研究奠定了良好基础。In this study, recombinant expression plasmid pVAX1/TSOL18 was constructed and then transfected into BHK-21 cell line with liposome 2000. The results of SDS-PAGE, Western blotting and fluorescence antibody assay showed that TSOL18 protein was successfully expressed in BHK-21 cell line and could be recognized by positive serum against cysticercosis. Immunization in mice indicated that the expression vectors could efficiently induce the specific cellular and humoral immunoresponse, which laid a solid foundation for the further development of new vaccines of cysticercosis.

关 键 词：猪带绦虫 TSOL18基因 基因疫苗表达载体 免疫原性 

分 类 号：S852.734[农业科学—基础兽医学]