喹乙醇对小鼠肝脏和肾脏的毒性作用研究  

Toxic Effect of Olaquindox on Liver and Kidney in Mouse

作　　者：尹荣焕[1] 白文林[1] 吴长德[1] 王健民[1] 刘宝山[1] 邹德研[1] 方和春 刘丽霞[1] 何剑斌[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161 [2]辽宁省辽中县疾病预防控制中心,辽中110200

出　　处：《畜牧兽医学报》2008年第7期974-979,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：辽宁省教育厅青年基金资助项目(05L404)

摘　　要：按毒理学方法给小鼠灌喂不同剂量喹乙醇,探讨喹乙醇对小鼠肝、肾脏功能的影响。结果表明:随着喹乙醇剂量的增加,血液中AST、ALT活性及BUN、Cre含量均升高,CHE活性先升高再下降,血清TP、ALB含量则明显下降。这些变化表明蛋白质合成代谢减慢,分解代谢加强,引起动物生长速度减慢。剖检时肝脏肿大、瘀血,质脆易碎,1/10LD50组和1/5 LD50组肝细胞变性、坏死,肾小管上皮细胞高度肿胀、变性、脱落,肾小球囊腔变狭窄。电镜下1/5 LD50组小鼠肝细胞部分细胞质溶解,细胞器减少,线粒体受损;肾脏近曲小管上皮细胞胞质部分溶解,线粒体嵴有缺损,有液泡,肾小球足细胞部分融合,证实了喹乙醇对肝肾的器质性损害。Based on the method of toxicology, mice were administered with different dosage of olaquindox by intragastric administration in order to investigate the effect of olaquindox on function of liver and kidney. The results were as follows： With the increase of olaquindox dosage, both the activity of GOT, GPT and the content of BUN, Cre in blood stepped up, the activity of CHE stepped up at first, and then stepped down, the content of TP and ALB stepped down obviously in serum. These results indicated that there was a decrease in anabolism of protein, and increase in catabolism, which resulted in a decrease in growth rates. At autopsia, the livers displayed swelling, blood stasis, and become breakable. Both denaturation and necrosis of liver cell were observed in 1/10 LD50 and 1/5 LD50 groups. The swelling, denaturation and desquamation was also observed in epithelia of nephridial tubule, at the same time, the glomerular capsule become incommodious. By electron microscope, plasmarrhexis, decrease of organelle, impairment of mitochondria were observed in partial liver cells in 1/5 LD50 group. Plasmarrhexis occurred in epithelia of proximal convoluted tubule, and impairment, vacuole appeared in mitochondrial cristae, while, there was some part fusion in Sertoli cells of glomeruli. These results confirmed the organic impairment of olaquindox on liver and kidney in mouse.

关 键 词：喹乙醇 肝功能酶 肾脏 毒性 

分 类 号：S856.9[农业科学—临床兽医学]