鬼臼多糖对小鼠免疫功能和抗氧化能力的影响  

Effects of Podophyllum Emodi Polysaccharide on Immune Function and Antioxidant Capability in Mice

作　　者：帅学宏[1] 胡庭俊[2] 陈炅然[3] 高丽萍[4] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]广西大学动物科学技术学院,南宁530005 [3]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [4]兰州大学基础医学院,兰州730000

出　　处：《畜牧兽医学报》2008年第7期1000-1004,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30371056);广西大学科研基金资助项目(X071026)

摘　　要：分离提取鬼臼多糖(PEP),进行理化性质检测、纸层析和红外光谱分析;体外培养小鼠脾脏淋巴细胞,测定了不同浓度PEP对淋巴细胞增殖的影响;观察了PEP(200 mg/kg体重)对小鼠脾脏指数、胸腺指数和溶菌酶含量的影响;选择地塞米松诱导的免疫功能低下模型小鼠,腹腔注射PEP(200 mg/kg体重)后测定T-SOD、MDA、T-AOC活力、O2.-活力和H2O2水平,观察了PEP对活性氧水平的影响。结果表明,PEP提取获得率为6.38%,不含淀粉、氨基酸和蛋白质,由葡萄糖、果糖、甘露糖和阿拉伯糖组成,红外光谱扫描呈现多糖类物质的特征吸收峰。药理试验表明,PEP对淋巴细胞体外增殖有促进作用(P<0.05);PEP可显著提高脾脏指数(P<0.05),可极显著对抗由地塞米松所致的T-SOD活力下降(P<0.01),能极显著降低血浆中MDA含量(P<0.01),极显著提高血浆中T-AOC能力(P<0.01),极显著提高脾脏抗超氧阴离子活力(P<0.01),降低H2O2含量(P<0.01)。A water-soluble polysaccharide from root of Podophyllum Emodi was obtained through boiling water. The physical and chemical characteristics of the Podophyllum Emodi polysaccharides（PEP） was analyzed by filter paper chromatography and FT-IR. Stimulation test of PEP on splenic lymphocyte proliferation was performed in vitro. In vivo,on day 1,3,5 and 7, ten mice were given PEP at dose of 200 mg/kg BW by abdominal injection. The model of immune hypofunction was established by using dexamethasone, PEP（200 mg/kg BW） was given to investigate the effects of the polysaccharide on immune function and oxidative stress. The results showed that PEP is composed of glucose, fructose, mannose and arabinose. PEP stimulated the lymphocytes proliferation and induced the lymphocyte proliferation in cooperation with ConA in vitro. PEP improved the spleen index（P〈0.05）. PEP can significantly increase the descent activity of T-SOD and activity of anti-superoxide anion induced by dexamethasone（P〈0.01）, and decreased the content of hydrogen dioxide and content of malondialdehyde（P〈0. 01）, it can also significantly increase the activity of T-AOC（P〈0. 01）in vivo. It is concluded that the PEP may play an important role in modulating immune function and prevention of oxidative damage in immunological system.

关 键 词：鬼臼多糖 理化性质 免疫 淋巴细胞 活性氧 

分 类 号：S852[农业科学—基础兽医学]