大蚕蛾科绢丝昆虫线粒体基因组A+T丰富区序列及其系统发育研究  

Study on nucleotide sequences in A+T-rich regions of mitochondrial DNAs from silkmoths of saturniidae and their molecular phylogeny

作　　者：沈兴家[1] 陈丽媛[1] 唐顺明[1] 孙孝龙[2] 赵巧玲[1] 朱晨[1] 郭锡杰[1] 

机构地区：[1]江苏科技大学 [2]盐城生物工程高等职业技术学校,江苏盐城224731

出　　处：《江苏科技大学学报（自然科学版）》2008年第3期66-71,共6页Journal of Jiangsu University of Science and Technology:Natural Science Edition

基　　金：国家重点基础研究发展计划"973"资助项目(2005CB121000)

摘　　要：为了研究大蚕蛾科绢丝昆虫的进化关系,分别从蓖麻蚕、樗蚕和柳蚕的蛹中提取线粒体DNA,用PCR方法扩增线粒体A+T丰富区及侧翼序列,克隆到pMD18-T载体,进行序列测定.序列分析表明,克隆片段的基因依次为12SrRNA基因3′端、A+T丰富区、tRNAMet、tRNAIle、tRNAGln和ND2基因5′端,与家蚕和野桑蚕mtDNA排列顺序相同;在tR-NAGln和ND2基因之间有一段非编码区.根据柳蚕3个tRNA基因的核苷酸序列推定了其二级结构.基于5种大蚕蛾科绢丝昆虫和家蚕mtDNA A+T丰富区序列的系统发育树显示,柳蚕与蓖麻蚕、樗蚕的亲缘关系较近,与柞蚕的亲缘关系较远,而与家蚕的亲缘关系更远.本研究结果有助于阐明大蚕蛾科绢丝昆虫以及家蚕的起源和进化.To study the phylogeny of silkmoths of Saturniidae, the mitochondrial DNAs （mtDNAs） were extracted from the pupa of Philosamia cynthia ricini ,Philosamia cynthia and Actias selene ningdoana respectively. The A ＋T-rich regions and flanking sequences were amplified with the polymerase chain reaction （PCR） method, and cloned into the pMD18 - T vector and then sequenced. Results from sequence analysis show that gene orders in all cloned fragments are 3＇ -end portion of 12S rRNA, A ＋ T- rich region, tRNA^Met, tRNA^Ile, tRNA^Gla and 5＇ - end portion of ND2, which are the same as those of Bombyx mori and Bombyx mandarina. Between tRNAGIn and ND2 there is a non - coding region. The secondary structures of three tRNAs of A. s. ningdoana are deduced based on the nucleotide sequences. The phylogenetic tree based on the A ＋ T-rich regions from 5 species of Saturniidae and 2 varieties of B. mori reveals that A. s. ningdoana is closer to P. c. ricini and P. c. cynthia, a little far from Antheraea pernyi, but remote to B. mori in blood relationship. These results will benefit to the study of the origins and the molecular evolution of Saturniidae insects as well as B. mori.

关 键 词：大蚕蛾科 非家蚕 线粒体DNA A+T丰富区 系统发育树 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]