环孢菌素抑制钙离子介导的HL-60细胞凋亡(英文)  

Cyclosporine inhibited calcium-mediated apoptosis of HL-60 cells

作　　者：黄其擎[1] 方敏[1] 张鸿卿[1] 薛绍白[1] 

机构地区：[1]北京师范大学生物系

出　　处：《中国药理学报》1997年第3期262-265,共4页Acta Pharmacologica Sinica

基　　金：Project supported by the National Natural Scrence Fundation of China(№ 39380024)

摘　　要：目的:研究环孢菌素(Cyc)在HL-60细胞凋亡中的作用.方法:通过DNA凝胶电泳、细胞形态观察及流式细胞术等方法确定药物三尖杉酯碱(Har), 喜树碱(Cam)或卡西霉素(Cal),thapsigargin(Tha)诱导的细胞凋亡.流式细胞术测定细胞凋亡中胞内相对自由钙浓度的变化.结果:Cal或Tha均能诱导HL-60细胞凋亡,其效应可被Cyc抑制;但Cyc不抑制Hat或Cam诱导的HL-60细胞凋亡.Cal或Tha均能诱导细胞内Ca^(2+)浓度升高,而Hat或Cam并不诱导胞内Ca^(2+)浓度升高.结论:Cyc只抑制由Ca^(2+)升高介导的细胞凋亡.Cal或Tha诱导的细胞凋亡机制不同于由Hat或Cam所诱导的细胞凋亡机制.AIM: To study the effects of cyclosporine (Cyc) on apoptosis of HL-60 cells. METHODS: Apoptotic cells induced by harringtonine (Har), camptothecin (Cam), or calcimycin (Cal), thapsigargin (Tha) were identified with DNA electrophoresis, morphology, and flow cytometry. Relative [Ca2+]; alteration of apoptotic HL-60 cells were determined with flow cytometry. RESULTS: Cal 1 mg·L-1 or Tha 0.5 mg·L-1 induced apoptosis of HL-60 cells. This effect was inhibited by nontoxic concentration of Cyc 1 mg · L-1. Cyc did not inhibit Har- or Cam-induced apoptosis of HL-60 cells. Both Cal and Tha increased intracellular calcium, whereas Har or Cam did not. CONCLUSION: Cyc inhibited apoptosis only induced by calcium increasement in HL-60 cells. The mechanism of apoptosis induced by Cal or Tha was different from that by Har or Cam.

关 键 词：环孢菌素 钙 细胞凋亡 HL-60细胞 

分 类 号：R979.5[医药卫生—药品]