雌激素诱导小鼠腹腔巨噬细胞凋亡(英文)  

Estrogens induce apoptosis in mouse peritoneal macrophages

作　　者：张修武[1] 牛喜林[1] 郭兆贵[1] 

机构地区：[1]湖南医科大学分子药理研究室

出　　处：《中国药理学报》1997年第3期267-270,共4页Acta Pharmacologica Sinica

基　　金：Project supported by the National Natural Scrence Foundation of China, № 39370310

摘　　要：目的:研究雌激素诱导小鼠腹腔巨噬细胞凋亡的作用.方法:分离、培养小鼠腹腔巨噬细胞,在 培养基中加17-β-雌二醇和雌酮处理,琼脂糖凝胶电泳观察DNA片段.结果:17-β-雌二醇0.01-1μmol·L^(-1)和雌酮10-20μmol·L^(-1)均能以剂量依赖方式诱导巨噬细胞产生凋亡的典型形态学改变和特征性DNA片段,staurosporine、放线菌酮和他莫西芬能取消这种作用.结论:雌二醇和雌酮能诱导小鼠腹腔巨噬细胞凋亡,这一过程与蛋白激酶C的活化和合成新的蛋白质有关.AIM: To study whether estrogen might induce apoptosis in mouse peritoneal macrophages (MPM). METHOD: The MPM were isolated and incubated in culture medium containing 17-β-estradiol, estrone, or equal volume of 100 % ethanol as control. DNA fragmentation was visualized by agarose gel electrophoresis. RESULTS: 17-β-Estradiol 0.01-1 μmol · L-1 or estrone 10 - 20 μmol·L-1 elicited typical morphological apoptosis and DNA fragmentation in a concentration-dependent manner in MPM. Staurosporine (Sta) 0.01μmol·L-1, cycloheximide (Cyc) 1 mg·L-1, and tamoxifen (Tarn) 10 μmol·L-1 inhibited the DNA fragmentation induced by 17-β-estradiol 1 μmol · L-1 or estrone 20 μmol ·L-1. CONCLUSION: Elstradiol and estrone induced apoptosis in MPM.

关 键 词：雌二醇 细胞凋亡 巨噬细胞 

分 类 号：R977.12[医药卫生—药品]