甘草次酸钠对大鼠化学性腹膜炎的影响(英文)  

Effect of sodium glycyrrhetinate on chemical peritonitis in rats

作　　者：包金凤[1] 吴勇杰[2] 杨永健[3] 李新芳 

机构地区：[1]兰州医学院 [2]兰州医学院药理学教研室 [3]兰州医学院生药学教研室

出　　处：《中国药理学报》1997年第3期277-280,共4页Acta Pharmacologica Sinica

摘　　要：目的:研究甘草次酸钠(SG)的抗炎机制.方法: 制备大鼠腹膜炎模型,用放免法测PGE_2含量,Folin-酚试剂法测蛋白含量,邻苯三酚-NBT法测SOD活性,竞争性蛋白结合法测cAMP含量.结果:组胺致炎时,SG 10-20 mg·kg^(-1) im减少渗液量;角叉菜胶致炎时,SG 20 mg·kg^(-1) im使渗液量及其中PGF_2含量减少,SOD活性增加;花生四烯酸致炎时,SG 20 mg·kg^(-1)im抑制Neu中cAMP浓度下降,减少渗出液中PGE_2含量.SG也使三种腹膜炎渗出液中Neu计数和蛋白含量减少.结论:SG通过抑制Neu游走及PGE_2合成、降低毛细血管通透性、清除氧自由基而发挥抗炎作用.AIM: To study the anti-inflammatory mechanisms of sodium glycyrrhetinate (SG). METHODS: Rat chemical peritonitis was used. The protein content and prostaglandin E2 (PGE2 ) content in exudate were measured by Folin-phenol assay and RIA, respectively. SOD activity in neutrophils (Neu) was determined by pyrogallol-NBT colorimetry. cAMP content in Neu was detected by competitive protein binding assay. RESULTS: In peritonitis caused by histamine, SG 10 - 20 mg · kg-1 im reduced exudate volume and Neu counts, and 5 - 20 mg · kg-1 im lowered the protein content in exudate. In peritonitis induced by carrageenan, SG 20 mg·kg-1 im reduced exudate volume, Neu counts, protein content and PGE2 content in exudate, increased SOD activity in Neu, but did not affect β-glucuronidase release from Neu. In peritonitis induced by arachidonic acid, SG 20 mg· kg-1 im reduced Neu counts, protein content, and PGE2 content in exudate, and attenuated the reduction of cAMP level in Neu. CONCLUSION: SG exerts its anti-inflammatory action by lowering permeability of capillaries in inflammatory site, inhibiting Neu emigration and PGE2 biosynthesis, and scavenging oxygen free radicals.

关 键 词：甘草次酸 腹膜炎 

分 类 号：R285.5[医药卫生—中药学] R259.722[医药卫生—中医学]