荧光偏振免疫分析法研究17种磺胺类药物与抗体亲和力的构效关系  

Studies on Structure-activity Relationship of 17 Sulfonamides Binding to Antibody by Fluorescence Polarization Immunoassay

作　　者：王战辉[1] 张素霞[1] 丁双阳[1] 沈建忠[1] 

机构地区：[1]中国农业大学动物医学院兽药和毒性系,国家兽药残留基准实验室,北京100094

出　　处：《高等学校化学学报》2008年第6期1107-1111,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学杰出青年基金(批准号:30325032);国家自然科学基金(批准号:30471305)资助

摘　　要：应用4株磺胺二甲基嘧啶(SMZ)多克隆抗体和5种不同结构的荧光标记物(Tracer)建立了荧光偏振免疫分析(FPIA)检测SMZ的方法.讨论了4株多抗对SMZ的检测灵敏度和对17种磺胺类药物(SAs)的相对亲和力趋势,从药物分子二维结构上探讨了多抗对小分子抗原的识别能力.结果表明,3号多抗对SMZ的灵敏度最高,但是4株多抗对17种SAs的相对亲和力趋势相似,说明抗体对抗原的结合力大小与抗原的化学结构有关.Fluorescence polarization immunoassay for the determination of sulfamethazine was developed via four polyclonal antibodies against sufamethazine and five fluorescein-labeled sulfonamides（tracers）. The study mainly focuses on the relative affinity trend of four polycolnal antibodies toward 17 sulfonamides analogs and explaination of the recognition between antibody and drugs based on the two-dimensional structures of drug antigen. The results demonstrate that the polyclonal antibody（ No. 3 ） showed the most sensitivity for sulfamethazine, however, the four polyclonal antibodies presented a similar relative affinity trend to 17 sulfonamides, implying that the affinity of antibody to antigen has correlationship with the chemical structure of. antigen.

关 键 词：荧光偏振免疫分析 磺胺类药物 多克隆抗体 构效关系 

分 类 号：O657.3[理学—分析化学]