不同介质中蜂毒素聚集/解聚集的超分子调控及相关机理  

Supramolecular Monitoring and the Related Mechanism of Aggregation/Disaggregation of Melittin in Different Media

作　　者：王磊[1] 郝雅琼[1] 李岳[1] 李正强[2] 吴玉清[1] 

机构地区：[1]吉林大学超分子结构与材料国家重点实验室,长春130012 [2]吉林大学分子酶学工程教育部重点实验室,长春130021

出　　处：《高等学校化学学报》2008年第6期1112-1115,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20473028,20773051);教育部新世纪优秀人才支持计划;国家“九七三”计划(批准号:2007CB808000);长江学者创新团队发展计划(批准号:IRT0422);高等学校学科创新引智计划(111)资助

摘　　要：利用荧光光谱和圆二色谱等技术手段研究了蜂毒素在2-羟丙基环糊精、氯化钠或DOPC调控下的聚集/解聚集过程、相关机理以及不同介质中α-helix的含量.研究结果表明,蜂毒素在不同介质中的聚集能力、构象以及和介质分子相互作用力均存在很大差别.Melittin, with strong pharmacological effects and biological activity, is the major component of bee venom. The physiological function of melittin depends upon its secondary structure and aggregation state due to which the peptide loses its activity. Once the aggregation/disaggregation processes can be effectively monitored, it will lay the foundation for clinical applications by exploring its function and mechanism. In this paper the aggregation/disaggregation processes were explored, the related mechanism and the α-helix content of melittin in aqueous solution is controlled by 2-hydroxypropyl-β-cyclodextrin, sodium chloride or 1,2-dioleoylsn-glycero-3-phosphocholine （ DOPC ） through fluorescence spectra and circular dichroism （CD）.

关 键 词：蜂毒素 2-羟丙基环糊精 氯化钠 DOPC Α-螺旋 

分 类 号：O657[理学—分析化学]