水包油包固体乳化法制备蛋白药物缓释微球  

Preparation of Controlled-release Microspheres Loading Protein Through Solid-in-oil-in-water Emulsion Method

作　　者：杜青[1] 胡俊丽[1] 韩亚冬[1] 陈学思[1] 景遐斌[1] 

机构地区：[1]中国科学院长春应用化学研究所高分子物理与化学国家重点实验室

出　　处：《高等学校化学学报》2008年第6期1262-1266,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学杰出青年基金(批准号:50425309);A3国际合作重大基金(批准号:20621140369);科技部国际合作重点项目(批准号:0760011B01)资助

摘　　要：以牛血清白蛋白(BSA)为模型药物,研究了一种新型载药微球的水包油包固体(S/O/W)乳化法.用纳米尺寸的SiO2吸附溶液中的BSA,得到粒径约为30nm的含药粒子,再用PLGA包裹含药粒子.考察不同制备条件对载药量和包封率的影响,并与传统的双乳法(W/O/W)进行了对比,发现该制备方法提高了药物的载药量(由2.5%到3.1%)和包封率(由72%到90%以上),同时提高了药物活性.A novel solid-in-oil-in-water（S/O/W） emulsion method for drug controlled-release system was investigated in this paper. Bovine serum albumin （BSA） was used as the model drug. At first, the nanoparticles containing proteins were obtained by adsorbing BSA from the solution onto the surface of nano-scale SiO2. Then PLGA microspheres loading the solid nanoparticles were fabricated with the solid-in-oil-in-water emulsion method. Compared with the traditional water-in-oil-in-water double emulsion method, this new method has marked advantages, such as improving the percentage of drug in microspheres and the drug entrapment efficiency. More importantly, the activities of protein could be retained better.

关 键 词：纳米二氧化硅 水包油包固体(S/O/W)乳化法 蛋白药物 药物缓释 

分 类 号：O633.14[理学—高分子化学]