RP—HPLC法同时测定白屈菜药材中血根碱、小檗碱和白屈菜红碱的含量  

Simultaneous determination of sanguinarine,berberine and chelerythrine in Chelidonium majus by RP-HPLC

作　　者：左金梁[1] 白璐[1] 宋欣鑫[1] 谷艳[1] 赵春杰[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2008年第6期903-905,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立白屈菜药材的质量标准。方法:采用高效液相色谱法,用 Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-1%三乙胺水溶液(25:75,三乙胺水溶液用磷酸调 pH 3)为流动相,流速:1 mL·min^(-1),检测波长263 nm,柱温:30℃。结果:血根碱在60～1200μg范围内线性良好,r=0.9996,平均加样回收率(n=3)为96.6%～99.9%,RSD 为0.6%～2.0%;小檗碱在30～600μg范围内线性良好,r=0.9999,平均加样回收率(n=3)为99.0%～104.1%,RSD 为0.8%～1.3%;白屈菜红碱在100～2000μg范围内线性良好,r=0.9998,平均加样回收率(n=3)为97.3%～100.8%,RSD 为0.7%～1 4%。结论:本试验同时测定白屈菜药材中血根碱、小檗碱、白屈菜红碱的含量,方法准确,灵敏,可靠。Objective ： To determine the content of sanguinarine, berberine and chelerythrine in Chelidonium majus. Methods ： The HPLC with a Diamonsil C 18 （250 mm× 4.6mm, 5 μm） was used. Acetonitrile - 1% triethylamine （25：75 ,adjusted to pH 3 with phosphoric acid） was used as mobile phase at the flow rate of 1 mL·min^-1 ,the detection wavelength was at 263 nm and column temperature was 30 ℃. Results ： The founded method linearity of sanguinarine was in the range of 60 to 1200 μg（r =0. 9996）. The average recovery rate（ n = 3 ）was 96.6% -99.9% with RSD of 0. 6% -2.0% ;that of berberine was in the range of 30 to 600 μg（r = 0. 9999）. The average recovery rate（n =3）was 99.0% - 104. 1% and RSD was 0. 8% - 1.3% ;and that chelerythrine was in the range of 100 to 2000 μg（r =0. 9998）. The average recovery rate（ n = 3 ） was 97.3% - 100. 8% and RSD was 0. 7% - 1.4%. Conclusion：The contents of sanguinarine, berberine and chelerythrine in Chelidonium majus were determinated simultaneouly. The method is accurate,sensitivite and reliable.

关 键 词：白屈菜药材 质量标准 血根碱 小檗碱 白屈菜红碱 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]