HPLC法测定燕子掌中3-甲基-2-丁烯基异胍的含量  

HPLC determination of 3-methyl-2-butenyl iso-guanidine in Crassula argentea

作　　者：魏志奇[1,2] 王福东 陈幸 汪汉卿 万尧德[1] 董小萍[2] 

机构地区：[1]四川省中医药研究所 [2]成都中医药大学药学院 [3]北京英福源生物技术有限责任公司

出　　处：《药物分析杂志》2008年第6期955-957,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立 HPLC 法测定燕子掌全草中3-甲基-2-丁烯基异胍的含量。方法:色谱柱为 Hypersil NH_2柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.2%磷酸水溶液(40:60),检测波长为220 nm,流速1.0 mL·min^(-1),进样量5 μL,柱温27℃。结果:3-甲基-2-丁烯基异胍进样量在0.042～0 380 μg范围内具有良好的线性关系(r=0.9998),回收率为98.9%～102.7%.RSD 为1.7%～2.2%(n=3)。结论:该法可靠性高,准确性和重复性好,适用于3-甲基2-丁烯基异胍的含量测定。Objective：To develop an HPLC method for determination of 3 -methyl -2 -butenyl/so -guanidine in Crassula argentea. Methods： The analytical column was a Hypersil - NH2 （4.6 mm× 250mm, 5 μm）. The mobile phase consisted of acetonitrile-0. 2% phosphoric acid solution （40： 60） ,the flow rate was 1.0 mL·min^-1. The injection volume was 5 μL. The detector wavelength was set at 220 nm. The column temperature was 27 ℃. Results ：3 - Methyl - 2 - butenyl/so - guanidine curve was linear over the range of 0. 042 - 0. 380 p,g （ r = 0. 9998 ）. The average recovery of the method was 98.9% - 102.7%, RSD was 1.7% - 2. 2% （ n = 3）. Conclusion ：The proposed method would be used in the quantitative analysis of 3 -methyl -2 -butenyl iso -guanidine and the method is convenient, economical and accurate with good reproducibility

关 键 词：高效液相色谱法 3-甲基-2-丁烯基异胍 燕子掌 

分 类 号：R917[医药卫生—药物分析学]