流动注射微乳液化学发光法测定盐酸氯米帕明  

Flow-injection microemulsions chemiluminescence analysis of clomipramine hydrochloride

作　　者：石文兵[1] 

机构地区：[1]长江师范学院化学系,涪陵408003

出　　处：《药物分析杂志》2008年第6期983-986,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：重庆市科技攻关计划项目(CSTC;2006AA7003);发光与实时分析重庆市重点实验室开放基金项目

摘　　要：目的:建立盐酸氯米帕明的化学发光测定新方法。方法:在酸性条件下,盐酸氯米帕明分子中氮原子被质子化后与阴离子 AuCl_4^-形成离子缔合物,该缔合物被二氯甲烷带入鲁米诺的氯化十六烷基三甲基铵反胶束微乳液中,离解出来的 AuCl_4^-立即与鲁米诺产生化学发光。在一定浓度范围内,发光强度与盐酸氯米帕明的含量呈线性关系,从而间接测定盐酸氯米帕明的含量。结果:在优化的试验条件下,线性范围为0.001～10 μg·mL^(-1),检出限(3σ)为0.03 ng·mL^(-1),对浓度为1.0 μg·mL^(-1)的盐酸氯米帕明进行11次平行测定,RSD 为2.6%。结论:该法可用于片剂、注射液和生物体液中盐酸氯米帕明的测定。Objective：To establish a chemiluminescence （CL） method for the determination of clomipramine hydro- chloride. Methods：In acidic condition, the nitrogen in clomipramine hydrochloride was easily protonated to form ion - complex with negative ion AuCl4^-. The ion - complex was extracted by dichloromethane, and entered a reversed micellar microemulsions of cetyltrimethylammonium chlorine containing luminol. The dissociated AuCl4^- reacted with luminol and produced an analytical chemiluminescence signal. Results：In the optimum conditions, the linear range was 0. 001 to 10 μg·mL^-1 and the limit of detection was 0. 03 ng ·mL^-1 with a relative standard deviation （n = 11 ） of 2.6% for 1.0 μg·mL^-1 clomipramine hydrochloride. Conclusion ：The method can be applied to the determination of the studied drug in tablets, injections and biological fliuds successfully.

关 键 词：微乳液 盐酸氯米帕明 化学发光 

分 类 号：R917[医药卫生—药物分析学]