赤鲜红褪色分光光度法测定马来酸氯苯那敏  

Fading spectrophotometric determination of chlorphenamine maleate with erythrosine

作　　者：周尚[1] 杨季冬[1,2] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]长江师范学院化学系,涪陵408100

出　　处：《药物分析杂志》2008年第6期987-989,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：重庆市教委资助项目(No.KJ051303);教育部春晖计划(Z2005-1-55001)

摘　　要：目的:建立赤鲜红褪色分光光度法测定马来酸氯苯那敏的含量。方法:在弱酸性介质中,马来酸氯苯那敏(CPM)与赤鲜红(ET)或曙红 Y(EY)阴离子借静电引力和疏水作用力而形成离子缔合复台物。结果:溶液的吸收光谱发生变化,赤鲜红体系发生明显的褪色作用,最大褪色波长位于525 nm,CPM 浓度在0 1～4.0 μg·mL^(-1)范围内遵从比尔定律,ε为3.5×10~4 L·mol^(-1)·cm^(-1)。结论:方法灵敏度较高,选择性好,操作简便快速,用于片剂及尿液中马来酸氯苯那敏的测定,结果满意。Objective：To determination the content of chlorphenamine maleate （CPM） by fading spectrophotometry. Method：In weak acidic medium,CPM react with halogenated fluoresce dyes such as erythrosine （ET） and eosin Y （EY） to form the ion -association complex through electrostatic and hydrophobic interaction. Results：The color of solutions changes obviously, erythrosine system has a remarkable color fading reaction at 525 nm, the con- centration of CPM obeyed Beer＇ law in the range of 0. 1 -4. 0μg· mL^-1 ,the molar absorptivity （ε） was 3.5 × 10^4 L ·mol^-1·cm^-1. Conclusion：The method is sensitive, simple and rapid, it has been applied to the determination of CPM in tablets and urine with satisfactory results.

关 键 词：马来酸氯苯那敏(CPM) 赤鲜红(ET) 曙红Y(EY) 分光光度法 

分 类 号：R917[医药卫生—药物分析学]