异丙酚对胎鼠离体海马神经元发育的影响  

Effect of propofol on development of hippocampal neurons of fetal rats in vitro

作　　者：彭书峻[1] 赵一凡[1] 王寿平[1] 潘永英[1] 王景峰[2] 陈倩茹[3] 彭俊[1] 王飞[1] 

机构地区：[1]中山大学附属第二医院麻醉科,广州市510120 [2]中山大学附属第二医院心内科 [3]中山大学附属眼科中心麻醉科

出　　处：《中华麻醉学杂志》2008年第5期448-451,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30772091）;广东省自然科学基金资助项目（05001737）

摘　　要：目的评价不同浓度异丙酚对胎鼠离体海马神经元发育的影响。方法SD大鼠妊娠18d后取胎鼠海马神经元，培养6d，调整细胞密度为1×10^5／ml，每孔100μl作为研究对象。本研究包括3个实验，各实验中研究对象均随机分为5组。实验一：每组10孔，C1组不做任何处理，E1组加入20％脂肪乳0．8μl／ml，P1,2,3，组加入异丙酚，终浓度分别为2、4和8μg／ml，分别于孵育4、8h时随机取5孔，洗脱、培养6d，测定神经元树突总长度、初级突起数和分支突起数；实验二：每组20孔，C2组不做任何处理，E2组加入20％脂肪乳2μl／ml，PⅠ,Ⅱ,Ⅲ。组加入异丙酚，终浓度分别为5、10和20μg／ml，孵育24h后洗脱，分别于培养2、4、6和8d时各随机取5孔，计数存活神经元。实验三：每组5孔，Q组不做任何处理，E3组加入20％脂肪乳5μl／ml，PⅠ,Ⅱ,Ⅲ组加入异丙酚，终浓度分别为5、10和50μg／ml，孵育24h，检测神经元凋亡率。结果实验一：与C1组比较，E1组神经元树突总长度、初级突起数和分支突起数差异无统计学意义（P〉0．05）；与E1组比较，P1,2,3组神经元树突总长度缩短、初级突起数和分支突起数减少，且呈浓度和孵育时间依赖性（P〈0．05）。实验二：与C2组比较，E2组神经元存活率差异无统计学意义（P〉0．05）；与E2组比较，PⅡ,Ⅲ组神经元存活率降低，神经元死亡发生时间及数量呈浓度依赖性（P〈0．05）；实验三：与C3组比较，E3组神经元凋亡率差异无统计学意义（P〉0．05）；与B组比较，PⅠ,Ⅱ,Ⅲ组神经元凋亡率均升高，且呈浓度依赖性（P〈0．05）。结论异丙酚通过抑制胎鼠海马神经元树突生长及促进神经元凋亡，抑制了神经元的发育，其程度与作用时间和浓度有关。Objective To evaluate the effect of different concentrations of propofol on the development of hippocampal neurons of fetal rats. Methods The hippocampal neurons were isolated from the fetuses of 10 Sprague Dawley rats after 18 d of gestation ,and cultured for 6 days. There were 1 × 10^5/ml neurons 100μl in each well. The study included three tests. The wells of neurons were divided into 5 groups in each test, Test Ⅰ ： There were 10 wells in each group; group C1 received no treatment; in group E1 20% lipid emulsion 0.8 μl/ml was added; in group P1.2.3 propofol was added at the final concentration of 2, 4 or 8 μg/ml respectively. The 5 wells of neurons were randomly selected at 4 or 8 h of incubation, and cultured for 6 d, for measurement of the total length of neuronal dentrite and number Of primary and branch neuronal processes using IBAS system. Test Ⅱ： There were 20 wells in each group; group C2 received no treatment; in group E2 20% lipid emulsion 2μl/ml was added; in group PⅠ,Ⅱ,Ⅲ propofol was added at the final concentration of 5, 10 or 20 μg/ml respectively. The 5 wells of neurons were selected after 24 h incubation for detection of the cell survival rate at 2, 4, 6 or 8 days of culture, Test Ⅲ ： There were 5 wells in each group; group C3 received no treatment; in group E3 20% lipid emulsion 5 μl/ml was added;in group PⅠ ,Ⅱ Ⅲpropofol was added at the final concentration of 5, 10 or 50 μg/ml i＇espeetively. The neurons were incubated for 24 h. The neuron apoptosis rate was detected using TUNEL cell staining. Results Test 1 ： there was no significant differenee in the total length of neuronal dentrite and number of primary and branch neuronal processes between group C1 and E1 （P 〉 0.05）. Propofol treatment significantly shortened the total length of neuronal dentrite and reduced the number of primary and branch neuronal processes in a concentration and intubation time dependent manner. Test Ⅱ ： there was no significant difference in the cell survival rate between g

关 键 词：二异丙酚 海马 神经元 发育障碍 

分 类 号：R977.1[医药卫生—药品] R749.16[医药卫生—药学]