三角褐指藻对黑暗胁迫的生理响应(英文)  

Physiological response of marine diatom Phaeodactylum tricornutum to darkness stress

作　　者：蔡卓平[1] 危蔚[2] 段舜山[1] 

机构地区：[1]暨南大学水生生物研究所,广州510632 [2]中国科学院广州能源研究所,广州510640

出　　处：《生态科学》2008年第1期6-11,共6页Ecological Science

基　　金：国家自然科学基金项目(30370231,30770336);广东省科技计划重点引导项目(2005B33201001);珠海市科技计划重点项目(PC20061045)

摘　　要：为了研究黑暗条件对三角褐指藻生长及生化组成的影响,并探讨三角褐指藻对黑暗环境的生长适应能力,我们对该藻进行12 d的黑暗处理,着重测定了藻细胞密度、生物量、叶绿素a、可溶性糖和蛋白含量等指标。结果表明,三角褐指藻对黑暗环境表现出一定的适应性忍耐能力,在12 d的长期黑暗胁迫条件下依然可以存活,而藻细胞生化组成对黑暗的响应程度很可能是该藻得以维持细胞低水平生长的主要原因。随着黑暗处理时间的延长,三角褐指藻生长状况受抑制的程度增大,其生长及生化组成与对照组相比发生了极显著的变化。实验结束时,黑暗处理下的藻细胞密度和生物量分别降低到2.93ⅹ105 cells.ml-1和0.011 g.ml-1,仅为对照的8.0%和37.3%。同样地,黑暗环境也明显地抑制了三角褐指藻体内生化物质的合成与积累,黑暗处理12 d时藻细胞的叶绿素a、可溶性糖和蛋白含量分别比对照降低了约89%、87%和85%。研究结果可为海洋微藻种质的筛选、种质资源库的构建及微藻生物资源的综合开发利用提供参考依据。In the present study, Phaeodactylum tricornutum was grown in laboratory under darkness condition for 12 days in order to investigate its growth and some chemical compositions in response to the darkness environment and to evaluate the adaptation of this economic microalga to darkness stress. The results indicated that Phaeodactylum tricornutum could still survive in the darkness condition, while darkness stress imposed significantly inhibitory influence on the growth of Phaeodactylum tricornutum during the course of the experiment. Algal cell density and biomass in the darkness treatment were reduced to 2.93×10^5 cells·ml^-1 and 0.011 g·ml^-1 on the day of termination of the experiment, only 8.0% and 37.3% of the control. Moreover, some chemical compositions including chlorophyll a, soluble sugar and protein were also apparently limited in the darkness condition, the contents of which were approximately 89%, 87% and 85% lower than those in the control on the 12^th day. Our results have provided supportive evidence that Phaeodactylum tricornutum tended to exhibit the capacity of sustaining in the darkness stress, which might be due to its pronounced ability in the biosynthesis and metabolism of protein, soluble sugar and chlorophyll a, but its growth performance was remarkably restricted over time. The findings presented here suggested that the adaptive ability of Phaeodactylum tricornutum to darkness stress should be considered into the core collection and screening, as well as comprehensive exploiture ofmicroalgal resources.

关 键 词：生长 生理响应 黑暗胁迫 三角褐指藻 

分 类 号：Q178.1[生物学—水生生物学]