固相萃取高效液相色谱法测定人血浆中依那普利浓度  

SOLID PHASE EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF ENALAPRIL IN HUMAN PLASMA

作　　者：谭力[1] 袁倚盛[1] 张昕[1] 赵飞浪[1] 

机构地区：[1]南京军区南京总医院仪器分析室

出　　处：《药学学报》1997年第11期857-864,共8页Acta Pharmaceutica Sinica

摘　　要：建立了用固相萃取高效液相色谱法测定依那普利血药浓度的方法。色谱柱为２００ｍｍ×４６ｍｍ不锈钢柱，内填ＳｐｈｅｒｉｓｏｒｂＣ８（５μｍ），流动相为乙醇—水—１０％Ｈ３ＰＯ４—三乙胺（３０∶７０∶１５∶０１）；流速１０ｍｌ·ｍｉｎ－１，紫外检测波长２１５ｎｍ。血样用固相小柱预处理。此法线性范围２５～１５０ｎｇ·ｍｌ－１。最小检测浓度１５ｎｇ·ｍｌ－１，日内及日间误差＜８８％，平均回收率＞９１６％。A reversed phase high performance liquid chromatographic method utilizing solid phase extraction has been described for the determination of enalapril in human plasma. The C 18 sorbent cartridges were conditioned and plasma samples were applied, washed with 20 mmol·L -1 HCl (2×0 5 ml) and petroleum ether (boiling range 60～90℃) subsequently; and eluted with methanol (3×0 5 ml). The eluent was evaporated to dryness, reconstituted in 100 μl mobile phase and injected. Chromatographic separation was achieved on a Spherisorb C 8 column (200 mm×4 6 mm, 5 μm), with ethanol—water—10% H 3PO 4—triethylamine (30∶70∶1 5∶0 1) at a flow rate of 1 0 ml·min -1 . UV detection was set at 215 nm. The calibration ranges were 2 5～150 ng·ml -1 with regression coefficient of 0 997 and detection limit of 1 5 ng·ml -1 . The within day RSD and between day RSD were <8 73%, the recovery of method >91 6%. This method was applied to the pharmacokinetic analysis of enalapril in 8 human volunteers.

关 键 词：依那普利 固相萃取 高效液相色谱 含量测定 

分 类 号：R972.4[医药卫生—药品] R927.2[医药卫生—药学]