纳米铁材料对小鼠肺灌洗液中白细胞和羟脯氨酸的影响  

Effect of Nanosized Iron on Leukocyte and Hydroxyproline in Bronchoalveolar of Mice

作　　者：丁峰[1] 宋文华[1] 白茹[2] 刘艳[2] 张文佳[2] 张金洋[3] 高敏苓[1] 

机构地区：[1]天津工业大学环境工程系,天津300160 [2]天津理工大学环境科学与安全工程学院,天津300191 [3]上海交通大学环境科学与工程学院,上海200240

出　　处：《南开大学学报（自然科学版）》2008年第3期1-4,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(30771771);天津市自然科学基金(06YEJMJC05600)

摘　　要：小鼠采用非暴露式气管内注人法注入不同浓度纳米铁颗粒物悬液,在第10d 处死小鼠,并进行肺泡灌洗,测定肺泡灌洗液(BALF)中各项指标、肺脏器系数.纳米铁10mg·kg^(-1)bw^(-1)组肺脏器系数、白细胞数目和羟脯氨酸含量均高于对照组和纳米铁1mg·kg^(-1)bw^(-1)组,差异显著(P<0.01或 P<0.05),在本实验条件下,纳米铁对小鼠肺造成急性损伤作用,对生物体健康构成潜在威胁,为研究纳米铁对人体及环境的潜在影响奠定实验基础.Animal models were estabilished by injecting granule solution into the lung of rats through trachea. All rats were intratracheally instilled every three days. At the tenth day, all rats were sacrificed and their lungs were lavaged. The results in bronchoalveolar lavage fluid were examined. Lung/body coefficient was measured. Lung organ coefficient, Leukocyte count and Hydroxyproline（HYP） concentration of nanosized iron 10 mg · kg^-1bw^-1 group were higher than the control group and the group of nanosized iron 1 mg · kg^-1bw^-1（P〈0. 01 or P〈0. 05）. The result of morphological observation indicated that the rats in nanosized iron 10 mg · kg^-1bw^-1 group had the supreme injury of lungs. Under this experimental condition, the effect of lung injury is more severity in nanosized iron. The study affords a foundation of research on the potential influence of nanosized iron to the human and environment.

关 键 词：纳米铁 环境与健康效应 肺损伤 

分 类 号：R994.6[医药卫生—毒理学]