纳米二氧化钛对小鼠肺部生化指标的影响研究  

Study on Pulmonary Lesions Induced by Nanometer Titanium Dioxide in Mice

作　　者：宋文华[1] 张文佳[1] 张金洋[2] 刘艳[1] 丁峰[2] 高敏苓[3] 胡卫萱[1] 

机构地区：[1]天津理工大学环境科学与安全工程学院,天津300191 [2]上海交通大学环境科学与工程学院,上海200240 [3]天津工业大学环境工程系,天津300160

出　　处：《南开大学学报（自然科学版）》2008年第3期14-18,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(30771771);天津市自然科学基金(06YEJMJC05600)

摘　　要：为研究纳米二氧化钛对小鼠肺部的损伤,将小鼠采用非暴露式气管内注入法注入颗粒悬液建立动物模型,每隔2d 染毒一次,在第10d 处死小鼠,测定肺脏器系数及肺部组织中自蛋白、总蛋白、酸性磷酸酶和碱性磷酸酶的含量.结果显示:纳米 TiO_210 mg·kg^(-1)bw^(-1)组以上指标均高于对照组和其它实验组,且差异显著(P<0.01或 P<0.05);纳米 TiO_21 mg·kg^(-1)bw^(-1)组的白蛋白、总蛋白、酸性磷酸酶和碱性磷酸酶也均高于对照组和微米 TiO_2实验组,差异显著(P<0.01或 P<0.05);微米组除碱性磷酸酶与对照组相比有明显差异(P<0.01),总蛋白、白蛋白和酸性磷酸酶与对照组相比均没有明显差异.在本实验条件下,纳米 TiO_2颗粒对小鼠肺部损伤比微米级 TiO_2更严重,引起气血屏障的渗透性改变,肺泡细胞膜损伤或细胞发生死亡和溶解,以及早期纤维化,并表现出计量依赖性.Animal models were worked out by injecting granule solution into the lung of mice through trachea to study pulmonary lesions in mice. All mice were intratracheally instilled every three days. At the tenth day, mice were sacrificed and. Lung organ coefficient, albumin （ALB）, total protein （TP）, acid phosphatase （ACP） and Alkaline phosphatase （ALP） were measured. Among these five groups, all indexes of nano 10 mg · kg^-1bw^-1group were obviously higher than others（P〈0. 01 or P〈0. 05）. ALB, TP, ACP and ALP of nano 1 mg · kg^-1 bw^-1 group were higher than those of the control group, micon 10 mg · kg^-1bw^-1 and micon 1 mg · kg^-1bw^-1 groups（P〈0. 01 or P〈0. 05）. ALP of micon 1 mg · kg^-1bw^-1 and micon 10 mg · kg^-1bw^-1 were markedly different from those of the control group, but other indexes of micon 1 mg · kg^-1bw^-1 and micon 10 mg · kg^-1bw^-1 had no obvious difference. Under this experimental condition, the lung scathe caused by nano-TiO2 was more serious than one caused by micon-TiO2. Nano-TiO2 included the change of osmosis of gas/blood barrier, alveolus membrane damnification or cell death and solution, alveolus epithelia damnification and lung forepart fibrosis. This study provided the basis for further studying mechanism of lung scathe caused by nano-TiO2.

关 键 词：纳米二氧化钛 总蛋白 白蛋白 酸性磷酸酶 碱性磷酸酶 

分 类 号：R994.6[医药卫生—毒理学]