多壁碳纳米管对小鼠肺部急性损伤的研究  

Study on Pulmonary Acute Lesions Induced by MWNT in Mice

作　　者：宋文华[1] 丁峰[1] 白茹[2] 张文佳[2] 刘艳[2] 张金洋[3] 高敏苓[1] 

机构地区：[1]天津工业大学环境工程系 [2]天津理工大学环境科学与安全工程学院,天津300191 [3]上海交通大学环境科学与工程学院,上海200240

出　　处：《南开大学学报（自然科学版）》2008年第3期19-23,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(30771771);天津市自然科学基金(06YEJMJC05600)

摘　　要：小鼠采用非暴露式气管内注人法注入不同浓度多壁碳纳米管(MWNT)颗粒物悬液,在第10d 处死小鼠,并进行肺泡灌洗,测定肺泡灌洗液(BALF)中各项指标、肺脏器系数,并进行病理形态学观察.MWNT 10mg·kg^(-1)bw^(-1)组以上各指标均高于对照组和纳米1mg·kg^(-1)bw^(-1)组,差异显著(p<0.01或 P<0.05);病理形态学观察结果表明,MWNT 10 mg·kg^(-1)bw^(-1)组小鼠肺部损伤最重.在实验条件下,MWNT 对小鼠肺造成急性损伤作用,对生物体健康构成潜在威胁,为研究 MWNT 对人体及环境的潜在性影响奠定实验基础.Animal models were worked out by injecting granule solution into the lung of rats through trachea. All rats were intratracheally instilled every three days. At the tenth day, all rats were sacrificed and their lungs were lavaged. The indexes in bronchoalveolar lavage fluid were examined. Lung/body coefficient was measured. Morphological observations were preformed. All results of MWNT 10 mg · kg^-1bw^-1 group were obviously higher than the control group and the group of MWNT 1 mg · kg^-1bw^-1（P〈0.01 or P〈0. 05）. The result of morphological observation indicated that the rats in MWNT 10 mg · kg^-1 bw^-1 group had the supreme injury of lungs. Under this experimental condition, the effect of lung injury is more severity in MWNT. The study affords a foundation of research on the potential influence of MWNT to the human and environment.

关 键 词：多壁碳纳米管(MWNT) 环境与健康效应 肺损伤 

分 类 号：R994.6[医药卫生—毒理学]