磁性三氧化二铁纳米粒子对HepG2细胞增殖及凋亡的影响  

Study on Proliferation Inhibition and Apoptosis Induction of Magnetic Fe_2o_3 Nanoparticles on HepG2 Cells

作　　者：夏婷[1] 唐萌[1] 殷海荣[1] 陆敏玉[1] 王姝[1] 张婷[1] 杨扬[1] 张宇[2] 顾宁[2] 

机构地区：[1]东南大学公共卫生学院,江苏南京210009 [2]东南大学生物科学与医学工程学院,江苏南京210096

出　　处：《南开大学学报（自然科学版）》2008年第3期29-33,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家重点基础研究发展计划973项目(2006CB705602);国家自然科学基金(30671782);国家863计划项目(2004AA302G40)

摘　　要：应用 HepG2细胞作为研究对象,探讨 Fe_2O_3纳米粒子对肿瘤细胞的活力与凋亡的影响.采用四甲基偶氮唑盐(MTT)还原检测法确定受试物的染毒剂量.分别对各个组进行流式细胞术检测细胞线粒体膜电位变化及凋亡.结果显示,Fe_2O_3纳米粒子在0.555～3.310 mg/mL 范围内均可抑制 HepG2细胞增值,IC_(50)为1.1lmg/mL.Fe_2O_3纳米粒子在0.173、0.347、0.694 mg/mL 剂量组均导致细胞线粒体膜电位均较对照组有所下降且细胞凋亡率显著增高.The adverse effects of proliferation and apoptosis and the oxidative damage on HepG2 cells induced by Fe2O3 nanoparticles were studied. The cytotoxicity and the intervening concentration of nanoparticles were detected by MTT reduction assay. The mitochondrial membrane potent （MMP） and cell apoptosis rate of different exposure group were analyzed by fluorospectrophotometry, respectively. The results indicated that the proliferations of HepG2 cells were suppressed when exposed to Fe2O3 nanoparticles of 0. 555-3. 310 mg/mL. The IC50 of Fe2O3 nanoparticles on HepG2 cells was 1. 11 mg/mL. The flow cytometry assay showed that the MMP decreased in different exposure groups and cell apoptosis rate of exposure groups were higher than that of the control group. It can be concluded that Fe2O3 nanoparticles could reduce the MMP and lead to apoptosis in HepG2 cells.

关 键 词：氧化铁磁性纳米颗粒 人肝癌细胞株 线粒体膜电位 细胞凋亡 

分 类 号：R994.6[医药卫生—毒理学]