变性梯度凝胶电泳(DGGE)技术在土壤微生物多样性研究中的应用  

Application of Denaturing Gradient Gel Electrophoresis to the Study of Soil Microbial Diversity

作　　者：辜运富[1] 张小平[1] 涂仕华[2] 

机构地区：[1]四川农业大学资源环境学院微生物系,四川雅安625014 [2]四川省农业科学院土壤肥料研究所,成都610066

出　　处：《土壤》2008年第3期344-350,共7页Soils

摘　　要：应用变性梯度凝胶电泳(DGGE)谱图分析技术对土壤微生物多样性进行描述,可以克服传统微生物分离纯化培养方法和显微技术的局限性。本文介绍了变性梯度凝胶电泳指纹图谱分析方法及其在土壤微生物多样性中的应用,包括对土壤特殊微生物生理类群,自然环境条件下微生物多样性变化,污染土壤微生物的多样性,不同种植制度下的土壤微生物多样性,转基因植物、微生物介入的土壤微生物多样性等方面的研究。Using DGGE fingerprint technique to describe and characterize soil microbial diversity helps overcome the limitation of the traditional techniques as it does not require removal and culture of the microbes. A brief introduction was presented to the DGGE fingerprint technique and its application to the study on soil microbial diversity, including the study on specific soil microbial community, soil microbe diversity in natural environment, in polluted soils, in different cropping systems, as well as in soils intervened with transgenic plants and microbes.

关 键 词：变性梯度凝胶电泳 16SrDNA PCR 土壤微生物多样性 

分 类 号：Q938.1[生物学—微生物学]