HPLC法测定抗生素S632-A中9-甲基链霉戊二酰亚胺的含量  

Determination of 9-methylstreptimidone in S632-A by HPLC

作　　者：徐榕[1] 吴香玫[1] 赵春燕[1] 王玉凤[1] 李电东[1] 蔡年生[1] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所,北京100050

出　　处：《中国新药杂志》2008年第12期1051-1052,1075,共3页Chinese Journal of New Drugs

摘　　要：目的：建立测定抗生素S632-A中9-甲基链霉戊二酰亚胺（S632-A2）含量的HPLC方法。方法：采用Agilent TC-C18柱（150 mm×4.6 mm,5μm）,流动相为甲醇-水（45∶55）;流速：0.6 mL.min-1,检测波长233 nm,柱温为室温。结果：S632-A2进样量在0.88~8.76μg范围内呈良好的线性关系（r=0.999 5）,回收率为99.97%,RSD为0.33%（n=9）。结论：此方法准确,重复性好,可为评价抗生素S632-A提供可靠依据。Objective：To develop a HPLC method for the determination of 9-methylstreptimidone in S632- A. Methods：The samples were separated on an Agilent TC-C18 column （150 mm × 4.6 mm,5 μm）. The mobile phase consisted of methanol-water （45： 55）. The flow rate was 0.6 mL-min-1 The detection wavelength was 233 nm. Results ： The linear range of 9-methylstreptimidone was 0.88 - 8.76 μg（ r = 0. 999 5 ）. The average recovery of assay was 99.97% with RSD of 0.33 % （n = 9）. Conclusion ： The method is accurate, reproducible and can be used to evaluate the quality of S632-A.

关 键 词：戊二酰亚胺抗生素 链霉菌S632 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R979.14