异位、在位和正常子宫内膜基质细胞分泌RANTES水平比较  

Comparison of RANTES expression in ectopic,eutopic and normal endometrium

作　　者：方春丽[1] 王秀丽[1] 王媁[1] 孔娜[1] 韩素萍[1] 

机构地区：[1]南京医科大学第一附属医院妇产科,江苏南京210036

出　　处：《南京医科大学学报（自然科学版）》2008年第7期831-835,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(30600671);江苏省自然科学基金资助(BK2006577)

摘　　要：目的:比较子宫内膜异位症患者异位内膜、在位内膜和正常子宫内膜分泌RANTES的水平,以了解盆腔局部趋化巨噬细胞浸润的始动因素。方法:不同浓度(5、10、20ng/ml)的IL-1β刺激培养异位内膜基质细胞;选择最佳刺激浓度的IL-1β刺激培养异位内膜、在位内膜和正常内膜基质细胞,在不同的时间点取培养上清ELISA检测RANTES水平。结果:10ng/ml的IL-1β是刺激异位内膜基质细胞分泌RANTES的最佳浓度;异位内膜基质细胞分泌RANTES的水平显著高于在位内膜和正常内膜(P<0.01);刺激培养60h后,在位内膜RANTES分泌量较正常内膜显著增加(P<0.05)。结论:EMS患者的在位内膜与正常内膜相比,其生物学特性已经发生了改变,盆腔内的异位内膜、巨噬细胞和细胞因子形成了一个炎症反馈环,促进了异位内膜的种植、生长和局部炎症的发生。Objective：To explore the major factor of enhancing monocyte recruitment into the peritoneal cavity. Methods：The stromal cell of ectopic implants was exposed to various concentrations （5,10,20 ng/ml ） of IL-113 and the 10 ng/ml of IL-113 was co cultured with ectopic implants,eutopic and normal endometrium. Concentration of RANTES in the cell culture supernatant was measured with a sandwish ELISA. Results：The 10 ng/ml of IL-1β elicited the highest secretion of RANTES in ectopic implants. The RANTES secreted by the stromal cell of ectopic implants was increased more significantly than that in eutopic and normal endometrium （P 〈 0.01）. After 60 h, the stromal cell of eutopic endometrium secreted much more RANTES than normal endometrium （P 〈 0.05）. Conclusion：These findings are consistent with a feed-forward inflammatory loop among the activate macrophages, RANTES produc- tion by ectopic implants and further monocyte chemotaxis. We postulate that this pathway is activated in endometriosis, and the bioactivity of eutopic endometrium was different from normal endometrium.

关 键 词：子宫内膜异位症 异位内膜 在位内膜 RANTES 巨噬细胞 

分 类 号：Q786[生物学—分子生物学]