微粒体甘油三酯转运蛋白MTP-493G/T基因多态性与冠心病的研究  

The study on microsomal triglyceride transfer protein -493G/T gene polymorphism in non-diabetic patients with coronary heart disease

作　　者：李瑞洁[1] 朱铁兵[1] 倪国贞[1] 杨志健[1] 王连生[1] 曹克将[1] 马文珠[1] 

机构地区：[1]南京医科大学第一附属医院心脏科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第8期998-1002,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅项目资助(01KJB320006)

摘　　要：目的:探讨中国汉族人群微粒体甘油三酯转运蛋白(microsomal triglyceride transfer protein,MTP)-493G/T基因多态性与冠心病患病风险的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,测定335例非糖尿病冠心病患者和206例无冠心病对照者MTP-493G/T基因多态性。比较两组MTP-493G/T的基因型与等位基因频率的差别,同时观察MTP-493G/T对血脂参数的影响。结果:在中国江苏汉族人群中发现三种基因型,GG型发生频率最高,GT型次之,TT型发生频率最低。冠心病组TT基因型频率(6%)高于对照组(3%)(P<0.05),且T等位基因频率(19%)亦明显高于对照组(12%)(P<0.01)。各基因型与冠状动脉病变支数无明显相关性。TT基因型TG、LDL-C水平高于GG型(P<0.05),而TC、HDL-C和LP(a)水平在两者间无统计学差异(P>0.05)。多因素Logistic回归分析显示TT基因型的OR值为2.14(95%CI1.22～6.56,P=0.014),是独立的危险因素,不依赖TT基因型升高TG、LDL-C的作用。结论:微粒体甘油三酯转运蛋白-493TT基因型可能与冠心病易感性有关,其作用不依赖于TT基因型对TG、LDL-C的影响。Objective：To investigate the relationship between the coronary heart disease and the microsomal triglyceride transfer protein （MTP）-493G/T gene polymorphism. Methods：There was a case-control study,which enrolled 335 non-diabetic patients with coronary heart disease （CHD） and 206 control subjects without CHD. The genotype and allele frequencies of MTP-493G/T gene polymorphism were detected by polymerase chain reaction-restricted fragments length polymorphism （PCR-RFLP）. Results：The highest frequency of MTP-493G/T genetic variation was GG genotype; the second was GT genotype; and the lowest was TT genotype in Han population of Jiangsu Province, China. Both the TT genotype frequency（6%） and the ＂T＂ allele frequency（19%） in the patients with CHD were higher than those in the control（3% and 12%）, respectively（P 〈 0.05）. But there wasn＇t a significant correlation between MTP-493G/T genotypes and the number of significantly diseased vessels. The TT genotype had the higher TG and LDL-C level（P 〈 0.05） than GG genotype. In multiple Logistic regression analysis,the TT genotype odds ratio for coronary heart disease was 2.14 （95%CI 1.22-6.56,P = 0.014）. Conclusion：The TT genotype of MTP-493G/T genetic variation might increase the risk of coronary heart disease, independent of lipid and lipoprotein factors.

关 键 词：冠状动脉疾病 限制性片段长度多态性 微粒体甘油三酯转运蛋白 

分 类 号：R541.4[医药卫生—心血管疾病]