硅胶为基质的疏水吸附剂的制备及其对牛血清白蛋白的吸附  

PREPARATION OF SILICA-BASED HYDROPHOBIC INTERACTION ADSORBENTS AND BSA ADSORPTIONS

作　　者：李玉龙[1] 孙彦[1] 李慕玲[2] 胡宗定[1] 

机构地区：[1]天津大学化学工程系,天津300072 [2]南开大学新技术实业集团公司,天津300071

出　　处：《离子交换与吸附》1997年第4期335-340,共6页Ion Exchange and Adsorption

基　　金：国家教委"跨世纪优秀人才培养计划"基金

摘　　要：以硅胶为基质，苯甲酰基为疏水配基，采用有机硅烷活化法制备疏水吸附剂。研究了牛血清白蛋白（BSA）的吸附平衡行为，探讨了活化时间、活化介质对硅胶活化程度的影响，测定了不同盐浓度下的平衡等温线，讨论了配基来源、温度对吸附剂疏水性的影响。Silica gels were activated by silanization with γ- aminopropyltriethoxysilane.Benzoic acid was immobilized to the activated gels to prepare hydrophobic interac tion adsorbents (HIA) for protein adsorption. The adsorption behavior of bovine serum albumin (BSA) on the activated gels and HIA, the effects of the activation time and the media used for activations, were studied. The adsorption isotherms of BSA on HIA could be expressed by Langmuir equation. Effects of ligand resources and temperature on the hydrophobicity of adsorbents were also discussed.

关 键 词：硅胶 疏水作用 吸附剂 牛血清白蛋白 层析法 HIC 

分 类 号：O658.110.3[理学—分析化学] Q512.103[理学—化学]