高效毛细管电泳-电化学检测对外消旋氯霉素前体的拆分研究  

Enantioseparation of the Prodrug of Chloramphenicol by Capillary Zone Electrophoresis with Electrochemical Detection

作　　者：方晓明[1] 刘晓峰[1] 叶建农[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《高等学校化学学报》1997年第12期1944-1948,共5页Chemical Journal of Chinese Universities

基　　金：中国科学院长春应用化学研究所电分析化学开放实验室资助

摘　　要：用毛细管电泳-电化学检测法对外消旋苏式-2-氨基-1-对硝基苯基-1，3-丙二醇（氯霉素前体）进行了拆分研究．考察了影响检测及分离效果的实验参数，同时对其拆分机制进行了初步探讨．Chiral separation of the enantiomers of threo-2-amino-1- (4-nitrophenyl )-1, 3-propanediol, namely the prodrug of chloramphenicol (PCP), was carried out by capil1aryzone electrophoresis with electrochemical detection employing β-cyclodextrin (β-CD) as achiral additive in strong alkaline solutions. The analytes were detected by electrochemistryusing a copper disk electrode at +675 mV versus Ag/AgCl reference electrode. Both the freeenantiomers and the enantiomer-cyclodextrin inclusion complexes could be detected usingthis approach, although the complexed forms gave lower oxidation currents than the uncomplexed ones. Factors affecting the chiral CE separation of the analytes, such as concentrationof running buffer and β-CD, applied voltage, were extensively investigated. Under the optimum conditions, baseline separation of the enantiomers could be accomplished in less than l8min. In addition, an attempt was made to elucidate the pIausible mechanism 0f the chiralrecognition.

关 键 词：毛细管电泳 电化学检测 手性 拆分 氯霉素前体 

分 类 号：R978.13[医药卫生—药品] O657.8[医药卫生—药学]