吖啶橙-细胞DNA荧光抑制法筛选抗癌药物  

Screening of Anticancer Drugs by Acridine Orange cellular DNA Fluorescence Inhibition

作　　者：唐宏武[1] 陈蓓[2] 陈观铨[2] 曾云鹗[1] 

机构地区：[1]武汉大学化学系,武汉430072 [2]武汉大学分析测试科学系,武汉430072

出　　处：《高等学校化学学报》1997年第12期1960-1962,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金;湖北省自然科学基金

摘　　要：A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was employed to trace the interaction of drugand cellular DNA. The results for five anticancer drugs, vincristine, cyclophosphamide,mustine, cis-platin and mitomycin-C are in accordance with the mechanism of the drugs binding to cellular DNA and this indicates that this method is suitable for screening cell cyclenonspecific anticancer drugs.A new method for preliminary screening of anticancer drugs by Hadamard transform microscopic fluorescence image analysis was proposed. Rat liver 2c hepatocyte was usedas the standard cell and acridine orange (AO) was employed to trace the interaction of drugand cellular DNA. The results for five anticancer drugs, vincristine, cyclophosphamide,mustine, cis-platin and mitomycin-C are in accordance with the mechanism of the drugs binding to cellular DNA and this indicates that this method is suitable for screening cell cyclenonspecific anticancer drugs.

关 键 词：CCNSA 筛选 荧光法 抗癌药物 吖啶橙 DNA 

分 类 号：R979.1[医药卫生—药品]