新疆紫草抗HIV有效成分咖啡酸四聚体的理化性质和纯化工艺研究  

Physicochemical stability and purification technology of caffeic acid tetramer from Arnebia euchroma

作　　者：李雅娟[1] 王隽妮 沙先谊[1] 方晓玲[1] 

机构地区：[1]复旦大学药学院,上海200032 [2]上海静安制药有限公司,上海200041

出　　处：《中国中药杂志》2008年第13期1552-1555,共4页China Journal of Chinese Materia Medica

基　　金：上海市科委西部合作项目(065458029)

摘　　要：目的:初步研究咖啡酸四聚体(CAT)的理化稳定性的基础上选择合适的大孔吸附树脂,去除杂质,提高紫草水溶性部分中有效成分咖啡酸四聚体的含量。方法:以咖啡酸四聚体为指标,HPLC检测纯化前后或其他条件改变的含量变化。结果:7种型号的树脂中LK001纯化效果最佳。确定工艺为吸附液质量浓度10 g·L^(-1),pH 4.5,3 BV·h^(-1)的流速流过树脂柱,用去离子水快速洗脱至Molish反应为阴性,再用20%乙醇洗脱至无色,然后以50 mL 45%的乙醇洗脱,洗脱液除去乙醇,50℃浓缩干燥得粉末。收率为3.6%,CAT单个有效成分的含量为58%,纯度提高了45倍。结论:采用LK001树脂可以去除杂质,提高粗提物中有效成分咖啡酸四聚体的含量。Objective： To purify caffeic acid tetramer （CAT） with macroporous resin on the basis of its fundamental physicochemical stability research. Method： The changes of CAT content were compared by HPLC method before and after the purification process, or while other conditions were altered. Result： LK001 was the best one among 7 kinds of macroporous resin in regard of purifying ability. The optimum absorbing technology was the solution concentration at 10 g ·L^-1 , pH at 4. 5, and the flow rate at 3 BV·h^-1. The best elufing technology was 45% ethanol as eluting agent, pH at 5.0, eluting volume at 50 mL after applying super-purified water＇and 20% ethanol. The yield of product was 3.6 percent, and the active compound CAT was 58 percent in the product. Conclusion： Macroporous resin LK001 is effective in enriching CAT from the crude extracts, thus this method of purification is advisable.

关 键 词：咖啡酸四聚体 大孔吸附树脂 HPLC 稳定性 纯化 

分 类 号：R284.1[医药卫生—中药学]