复方加味桃仁承气汤对小鼠胰岛β细胞作用的研究  

Effect of Supplemented Taoren Chengqi decoction on NIT-1,a pancreatic β-cell from a transgenic NOD/Lt mouse

作　　者：孟繁敏[1] 赵玉英[1] 王宏[2] 张岩松[1] 李清艳[1] 李丽英[3] 张庆英[1] 

机构地区：[1]北京大学药学院天然药物学系及天然药物与仿生药物国家重点实验室,北京100083 [2]北京大学干细胞研究中心,北京100083 [3]首都医科大学细胞生物学系,北京100069

出　　处：《中国中药杂志》2008年第13期1602-1604,共3页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30371735)

摘　　要：目的：研究复方加味桃仁承气汤对小鼠胰岛β细胞（NIT-1）的作用，探讨其抗糖尿病机制。方法：利用^3H—TdR掺入、细胞计数、胰岛素测定等研究复方加味桃仁承气汤水提液及含药血清对小鼠胰岛卢细胞的增殖及胰岛素分泌量的影响。其中含药血清的制备方法为给随机分组的5只大鼠灌胃复方加味桃仁承气汤水提液，每天2次，连续3d，末次给药2h后腹主静脉取血，离心，分离血清。结果：复方加味桃仁承气汤水提液及其含药血清均能明显促进小鼠胰岛β细胞增殖（P〈0．05），同时能明显促进胰岛素分泌（P〈0．05）。结论：复方加味桃仁承气汤抗糖尿病的作用机制可能与其促进胰岛β细胞增殖及胰岛素分泌有关。Objective： To study the effect of Supplemented Taoren Chengqi decoction （STCD） on the secretion of insulin and proliferation of NIT-1. Method. The effect of STCD and the serum of rat after orally administrating of STCD on the secretion of insulin and proliferation of NIT-1 were studied. The proliferation of NIT-1 was measured by ^3 H-TdR incorporation and cell counting methods, while the secretion of insulin was measured from the cultured medium by the ultra sensitive rat insulin ELISIA kit. Result： Both the STCD and the serum of rat after orally administrating of STCD significantly could increased the secretion of insulin and proliferation of NIT-1. Conclusion： The treatment of the diabetic patients by STCD might be through with its improvement of secretion of insulin and proliferation on pancreatic β-cell.

关 键 词：糖尿病 加味桃仁承气汤 小鼠胰岛Β细胞 

分 类 号：R285.5[医药卫生—中药学]