Schiff碱金属配合物在细菌代谢过程中的表征  

Characterization of Some Schiff Base Metal Complexes in the Metabolism of Aerobacter aerogenes

作　　者：冯英[1] 黄在银[1] 谢昌礼[1] 屈松生[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《化学学报》1997年第10期967-973,共7页Acta Chimica Sinica

基　　金：国家自然科学基金;高等学校博士点专项基金资助的课题

摘　　要：采用微量热法测定了产气杆菌在四种Schiff碱金属配合物作用下的代谢热谱,以其对数生长期的生长速率常数k表征了Schiff碱金属配合物对其细菌生长的抑制. 不同的配合物其抑制情况不同.Co(Ⅱ)-NG(NG:D-氨基葡萄糖-β-萘酚醛Schiff碱)对产气杆菌的抑制作用最大,随浓度c的增加抑制作用增大,在350μg·mL^(-1)时可完全抑制其生长,但k-c并不呈线性关系;Fe(Ⅱ)-NG对产气杆菌的抑制较小,且在50～300μg·mL^(-1)浓度范围内k与浓度无关,在350μg·mL^(-1)时才略表现有一定的抑制作用;Cu(Ⅱ)-NG和Zn(Ⅱ)-NG对产气杆菌的抑制作用也较小,且k随浓度的变化不规则.同时发现热谱上最大热输出功率P_(max)与生长速率常数存在正比关系,进而推出在实验条件相同的情况下可用P_(max)代替k.Microcalorimetry was used to determine the actions of some schiff base metal complexes on the Aerobacter aerogenes. Difference in their capacities to inhibite the metabolism of this bacterium, as judged from the rate constant k (in log phase), varied with the different schiff base metal complexes. The growth rate constants of the bacteria in the presence of Co( II ) - NG(NG: D - glucosamine - β - naphthol aldehyde) decreased with the increasing of its concentrations , but the relationship between k and c is not of good linearity . In the case of Fe( II )- NG the rate constants keep unvaried with the variation of its concentrations. It seems no rule in the inhibitory effect of Zn( II ) - NG,Cu( II ) - NG on Aerobacter aerogenes. We also found that the relation between k and Pmax (the maximal thermal power) is a good linear equation, so the k can be replaced by Pmax at the same experimental conditions.

关 键 词：微量热法 配合物 产气杆菌 钴 铁 铜 锌 席夫碱 

分 类 号：R378[医药卫生—病原生物学] R965.1[医药卫生—基础医学]