基于PEG凝集的压电免疫传感器用于日本血吸虫抗体的测定  

Piezoelectric Immunosensor Based on the Agglutination of PEG for Determination of S. Japonicum Antibody

作　　者：吴朝阳[1] 沈国励[1] 俞汝勤[1] 汪世平[2] 曾宪芳[2] 

机构地区：[1]湖南大学化学计量学与化学传感器技术研究所,长沙410082 [2]湖南医科大学,长沙410078

出　　处：《高等学校化学学报》1997年第11期1774-1778,共5页Chemical Journal of Chinese Universities

基　　金：世界卫生组织资助;高等学校博士点基金;湖南省自然科学基金

摘　　要：以PEG为乳剂，建立了一种新的液相压电免疫分析法，并用于日本血吸虫感染兔血清的测定．初步探讨了这种PEG液相压电免疫传感器的响应机理，详细考察了PEG浓度、PEG－AWA复合时间及测试温度等因素对频移的影响．对5个兔血样的测试结果表明，应用该方法不仅对日本血吸虫病的阳性判别率可达100％，而且能定量测定感染血清中血吸虫抗体的含量．A new liquid piezoelectric immunoassay was developed to determine infectionrabbit serum (IRS) by adult worm antigen (AWA ) of S. japonicum, which is based on thetination of antibody and antigen-PEG complex by an immunoreaction. By examining thesurface appearances of the quartz crystal with scanning electronic microscopy(SEM) technologyand investigating the effect of the density and viscousity of the solution, it can be found thatthe absorption on the surfaces of the quartz crystal plays a dominant role in the frequencyresponse in the immunoassay. The influences including PEG concentration, complexing time ofAWA-PEG complex and detection temperature on frequency responses were investigated indetail, respectively. The frequency shift was linear with the antibody concentration in therange of 3.6~42.0μg/mL. Five rabbit serum specimens were analyzed with this method andthe analytical results given by this approach show that the detection ratio of positive serumreaches 100% and the antibody concentration can be quantitatively determined.

关 键 词：液相压电 免疫分析 日本血吸虫抗体 血吸虫抗体 

分 类 号：R532.210.4[医药卫生—内科学] R446.62[医药卫生—临床医学]