多枝赖草Glutathione Reductase基因克隆及胁迫表达分析  

Cloning and Stress Expression Analysis of Glutathione Reductase Gene in Leymus multicaulis

作　　者：史仁玖[1] 郝岗平[1] 赵茂林[2] 杨清[3] 

机构地区：[1]泰山医学院生物科学系,山东泰安271000 [2]北京农业生物技术研究中心,北京100089 [3]南京农业大学生命科学学院,江苏南京210095

出　　处：《四川师范大学学报（自然科学版）》2008年第4期471-475,共5页Journal of Sichuan Normal University（Natural Science）

基　　金：国家自然科学基金(30370905)资助项目

摘　　要：为了获得完整的谷胱甘肽还原酶基因序列,根据已克隆到的谷胱甘肽还原酶cDNA片段设计引物,利用RACE扩增获得了基因全长序列,应用Southern印迹杂交法分析基因存在状态,Northern印迹杂交法研究基因表达情况.结果表明,该基因全长1580 bp,含一个1 140 bp的开放阅读框架,编码380个氨基酸,与其它植物谷胱甘肽还原酶氨基酸序列的同源性在77%-92%之间;Southern杂交表明该基因有一个拷贝;Northern杂交表明在逆境胁迫下GR基因表达加强.In order to obtain a complete glutathione reduetase （GR） gene sequence, according to the primers designed by the cloned eDNA fragment of glutathione reductase in Leymus multicaulis, 5＇eDNA and 3＇eDNA were obtained by RACE. This paper uses southern bolt hybridization method to analyze the gene existence state, and uses northern bolt hybridization method to study the gene expression. The results show that the gene is 1 580 bp in length, including an open reading, encoding 380 amino acids. Its sequences shared high similarity at polypeptide level with the sequences from the glutathione reductase of other plants. Southern blot analysis shows that GR gene may have a single-copy in Leymus multicaulis. Northern blot analysis indicates that the GR gene expression will be strong in bad condition stress.

关 键 词：多枝赖草 谷胱甘肽还原酶 盐胁迫 表达 

分 类 号：Q945[生物学—植物学]