益气化纤汤对博来霉素A_5诱导的大鼠肺纤维化的影响  

Effect of Yiqihuaxian Decoction on Bleomycin-induced Pulmonary Fibrosis in Rats

作　　者：郑洁[1] 刘良徛[1] 姜瑞姣 

机构地区：[1]江西中医学院附属医院呼吸科,南昌330006

出　　处：《江西中医学院学报》2008年第3期72-74,共3页Journal of Jiangxi College of Traditional Chinese Medicine

摘　　要：目的:研究益气化纤汤对博莱霉素A5(BLMA5)诱导的大鼠肺间质纤维化的治疗作用。方法:利用博莱霉素气管内注射制作大鼠肺纤维化模型。90只健康雄性SD大鼠被随机分为正常组、模型组、强的松组和小、大剂量益气化纤汤组。在同样条件下,药物灌胃饲养28d后,断颈处死。取双肺标本进行病理切片、测定转化生长因子(TGF-β1)表达和羟脯氨酸(HYP)含量。结果:从病理切片、肺组织中TGF-β1表达和肺组织匀浆中羟脯氨酸的含量方面来看,药物治疗各组的肺纤维化均比模型组轻。结论:益气化纤汤对博莱霉素诱导的大鼠肺纤维化有一定的治疗作用,其作用机制可能通过抑制TGF-β1生成实现。Objective： To study the inhibitory effects of Yiqihuaxian（YQHX） decoction on bleomycin-induced pulmonary fibrosis in rats Methods： Bleomycin was injected intratracheally to make rats lung fibrosis model. The rats were randomly divided into normal group, model group, prednisone group and YQHX decoction group（ small dose, large dose）. All rats were raised under the same conditions. After 28 days, the rats were killed and the lungs were examined pathologically and the expression of TGF-β1 and the content of hydroxyproline were determined. Results： The changes of pulmonary fibrosis reflected by lung pathological examination, TGF-β1 expression and hydroxyproline content were lighter in the drug therapy groups than those in the model group. Conclusion： YQHX decoction has a certain treating effect on the pulmonary fibrosis induced by bleomycin in rats, possibly through inhibition of TGF-β1 expression.

关 键 词：益气化纤汤 博来霉素 肺纤维化 TGF-Β1 羟脯氨酸 

分 类 号：R285.4[医药卫生—中药学]