苹果黑星病菌遗传多样性的SSR分析  

Genetic diversity of Venturia inaequalis populations based on SSR analysis

作　　者：胡小平[1] 董艳玲[1] 苟建军[1] 杨家荣[1] 

机构地区：[1]西北农林科技大学植保资源与病虫害治理教育部重点实验室,杨凌712100

出　　处：《植物病理学报》2008年第3期329-332,共4页Acta Phytopathologica Sinica

基　　金：欧盟科技合作项目(ICA4-CT-2001-10001);教育部长江学者和创新团队发展计划资助项目(200558);西北农林科技大学优秀人才资助项目(04ZR018)

摘　　要：Apple scab caused by Venturia inaequalis has a tendency to spread and threatens the development of apple production in recent years in China.The genetic diversity and population structure were investigated by using simple sequence repeat(SSR) markers.51 strains were classified into 3 groups by UPGMA method as Xunyi,Xingping and U.K.population,each of them mainly including strains from its original place.A relatively high level of genetic diversity was revealed: H=0.425 3,I=0.675 8,PPL=66.67%(at species level);H=0.149 1,I=0.228 0,PPL=44.44%(at population level).A high level of genetic differentiation was detected among/within populations with Nei’s Gst analysis and AMOVA.Molecular genetic variance within populations was greater than that among populations.Genetic variance among populations might result from barriers to gene flow(Nm=0.675 8).Genetic variance within populations might result from sexual pro-pagation of V.inaequalis.Apple scab caused by Venturia inaequalis has a tendency to spread and threatens the development of apple production in recent years in China. The genetic diversity and population structure were investigated by using simple sequence repeat （SSR） markers. 51 strains were classified into 3 groups by UPGMA method as Xunyi, Xingping and U. K. population, each of them mainly including strains from its original place. A relatively high level of genetic diversity was revealed： H =0.425 3, I = 0.675 8, PPL = 66.67% （at species level） ; H =0. 149 1, I =0. 228 0, PPL =44.44% （ at population level）. A high level of genetic differentia- tion was detected among/within populations with Nei＇ s Gst analysis and AMOVA. Molecular genetic variance within populations was greater than that among populations. Genetic variance among populations might result from barriers to gene flow （Nm =0. 675 8）. Genetic variance within populations might result from sexual propagation of V. inaequalis.

关 键 词：苹果黑星病 遗传多样性 黑星病菌 SSR分析 可持续发展 苹果产区 流行速度 苹果产业 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治] Q943[农业科学—植物保护]