C57BL/6哮喘小鼠细胞内镁含量与肺组织β_2受体mRNA表达的相关性研究  

Correlation between intracellular magnesium and expression of beta 2-adrenergic receptor mRNA in the lung of C57BL/6 asthmatic mice

作　　者：张蔚[1] 江山平[1] 李元广[1] 郭淑芳[1] 刘珊英[2] 潘秋辉[2] 林小玲[1] 

机构地区：[1]中山大学附属第二医院呼吸内科,广东广州510120 [2]中山大学附属第二医院医学研究中心,广东广州510120

出　　处：《中国病理生理杂志》2008年第7期1394-1398,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技计划项目重大科技专项资助项目(No.2002311);广东省自然科学基金资助项目(No.7001620)

摘　　要：目的:探讨细胞内镁含量对C57BL/6哮喘小鼠肺组织β2受体mRNA表达的影响。方法:健康4-6周龄清洁级雌性C57BL/6小鼠96只,体重(12±2)g,随机数字表法分为A、B、C、D4组,每组各24只。应用鸡卵蛋白(OVA)建立哮喘小鼠模型。A、B组予低镁饲料喂食,C、D组予正常镁饲料喂食。B、D组腹腔内注射沙丁胺醇诱导β2受体低调节,A、C组腹腔内注射生理盐水作对照。第1d、21d、34d各组分别随机抽取8只检测血浆Mg2+、红细胞内Mg2+、肺组织β2受体mRNA及蛋白表达。结果:1d血浆Mg2+、红细胞内Mg2+、肺组织β2ARmR-NA及蛋白表达各组间差异无显著(均P>0.05)。21d、34dC组血浆Mg2+、红细胞内Mg2+、肺组织β2ARmRNA及蛋白表达均显著高于A组[21d:(0.84±0.09)mmol/Lvs(0.57±0.10)mmol/L、(2.39±0.14)mmol/Lvs(2.11±0.08)mmol/L、(0.75±0.09)mmol/Lvs(0.59±0.06)pmol/g、(88.50±8.50)pmol/gvs(60.10±7.70)pmol/g,均P<0.05;34d:(0.67±0.10)mmol/Lvs(0.51±0.09)mmol/L、(2.17±0.08)mmol/Lvs(2.05±0.09)mmol/L、(0.61±0.05)pmol/gvs(0.53±0.06)pmol/g、(76.60±7.10)pmol/gvs(58.00±7.60)pmol/g,均P<0.05];21d、34dD组血浆Mg2+、红细胞内Mg2+、肺组织β2ARmRNA及蛋白表达亦显著高于B组[21d:(0.95±0.33)mmol/Lvs(0.46±0.09)mmol/L、(2.32±0.18)mmol/Lvs(1.87±0.14)mmol/L、(0.73±0.10)pmol/gvs(0.43±0.07)pmol/g、(96.90±8.00)pmol/gvs(47.90±4.90)pmol/g,均P<0.05;34d:(0.71±0.10)mmol/Lvs(0.31±0.08)mmol/L、(1.66±0.13)mmol/Lvs(1.45±0.16)mmol/L、(0.40±0.07)pmol/gvs(0.33±0.05)pmol/g、(61.50±3.20)pmol/gvs(35.30±7.10)pmol/g,均P<0.05]。结论:细胞内镁缺乏的C57BL/6哮喘小鼠肺组织β2受体表达减少,在激动剂作用下更易发生β2受体低调节。AIM ： To explore the effect of intracellular magnesium on expression of beta 2 - adrenergic receptor mRNA in the lung of C57BL/6 asthmatic mice. METHODS： Ninetysix healthy, 4 -6 weeks old and female C57BL/6 mice, weighting （ 12±2） g, were randomly divided into the following A, B, C, D groups with 24 mice in each group. The mice were sensitized and challenged by ovalbumin to establish the asthmatic model. A and B groups were fed with magnesium deficient diet. C and D groups were fed with normal magnesium level diet. B and D groups were injected intrapedtoneally with 0.2 mL sulphate salbutamol solution after OVA provocation. A and C groups were injected intraperitoneally with 0.2 mL saline as control. Eight mice in each group were randomly taken out at 1 d, 21 d, 34 d to detect plasma Mg^2＋ , intracellular Mg^2＋, the beta 2 - AR mRNA and protein in lung tissue. RESULTS： No significant difference in plasma Mg^2 ＋, intracellular Mg^2＋, the beta 2 - AR mRNA and protein in lung tissue among all groups at 1 st d was observed （ P 〉 0. 05, respectively）. Plasma Mg^2＋ , intraeellular Mg^2＋ , the beta 2 -AR mRNA and protein in lung tissue in group C at 21st d and 34th d were significantly higher than those in group A at 21 st d and 34th d [ 21st d：（0. 84 ± 0. 09）mmol/L vs 0.57±0. 10）mmol/L, （2.39 ±0.14）mmol/L vs （2. 11 ±0.08） mmol/L,（0.75 ±0.09）pmol/g vs （0.59±0.06） pmol/g, （88.50 ＋ 8. 50） pmol/g vs （ 60. 10±7.70 ） pmol/g, P 〈 0. 05, respectively ; 34th d- （ 0. 67 ± 0. 10 ） mmol/L vs （0.51±0.09）mmol/L, （2. 17 ＋0.08）mmol/L vs （2.05 ± 0. 09 ） mmol/L, （ 0. 61 ± 0. 05 ） pmol/g vs （0.53 ±0.06） pmol/g, （76. 60 ±7. 10）pmol/g vs （58. 00 ±7.60） pmol/g, P 〈0. 05, respectively]. Also, plasma Mg2＋ , intracellular Mg^2＋ , the beta 2 -AR, mRNA and protein of lung tissue in group D at 21st d and 34th d were significantly higher than those in group B at 21 st d and 34th d [ 21 st d ： （ 0. 95 ± 0. 33 ） mrnol/L vs （

关 键 词：镁 哮喘 受体 肾上腺素能Β2 

分 类 号：R562.25[医药卫生—呼吸系统]