金黄色葡萄球菌agrC的克隆及序列分析  

Cloning and Sequence Analysis of agrC Gene of Staphylococcus aureus Accessory Gene Regulator System Receptor Protein

作　　者：王军华[1] 权春善[1] 郑维[1] 李巍[2] 王英国[3] 范圣第[1] 

机构地区：[1]大连民族学院生物技术与资源利用国家民委-教育部重点实验室,辽宁大连116600 [2]大连理工大学生物科学与工程系,辽宁大连116024 [3]中国科学院大连化学物理研究所,辽宁大连116623

出　　处：《微生物学杂志》2008年第3期45-49,共5页Journal of Microbiology

基　　金：辽宁省自然科学基金(2040949)

摘　　要：金黄色葡萄球菌agr系统能够调控多种毒力因子的表达,在该系统中,受体组氨酸蛋白激酶AgrC感受外部信号并传递给细胞内,在整个agr系统中起着重要的作用,成为药物发现的新靶点,该蛋白由agrC基因编码。通过分析发现,agrC基因的保守性非常差,这可能是由于它作为受体需要与信号分子结合的特异性所决定的。通过对已经公开的agrC基因序列进行比对分析,设计了多条引物,以金黄色葡萄球菌ATCC 6538的基因组DNA为模板,进行了PCR扩增并转化大肠埃希菌克隆该基因,获得了金黄色葡萄球菌ATCC 6538附属基因调节系统agrC基因的全序列。同时,通过对现有的提取金黄色葡萄球菌基因组的方法做了改进,得到了一种大量提取了金黄色葡萄球菌DNA的方法,获得的DNA纯度较高。Accessory gene regulator （agr） system of Staphylococcus aureus （SA） could regulate the expression of many virulence factors. In the system, being affected by external signal the receptor histidine protein kinase AgrC transfers to inside the cell, it plays very important role in the whole agr system and become a new target for founding medicine, the protein is encoded by the agrC gene. It was found through analysis that the conservativeness of agrC gene is very poor, possibly because the determination of the specificity of it as a receptor that needs to combine with signal molecule. Through comparison and analysis reported agrC gene sequence, six primers were designed taking SA ATCC 6538 genome DNA as template and carried out PCR amplification, cloning of the agrC and transferred into E. coli, a complete accessory gene regulator system agrC gene sequence was obtained. Furthermore, a new method to extract SA DNA with high purity in large scale was gained through refining the existing methods for extracting SA genome.

关 键 词：金黄色葡萄球菌 DNA提取 克隆 附属基因调节系统 agrC 基因序列 

分 类 号：TU317[建筑科学—结构工程]