腹部开放伤合并人工海水浸泡大鼠肠道免疫屏障功能的变化  

Changes of intestinal immune function in rats with open celiac seawater immersion wounds

作　　者：刘刚[1] 王强[1] 王育红[2] 周丽君 王欲晓 

机构地区：[1]第二军医大学附属长征医院普通外科,上海200003 [2]海军总医院普通外科 [3]海战伤救治研究中心

出　　处：《中华航海医学与高气压医学杂志》2008年第3期131-134,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的探讨腹部开放伤合并人工海水浸泡大鼠肠道免疫屏障功能的变化及意义。方法建立腹部开放伤合并人工海水浸泡大鼠致伤模型。50只Wistar大鼠随机分为5组，每组10只。A组：腹部开放伤合并海水浸泡组；B组：单纯腹部开放伤组；C组：单纯海水浸泡组；D组：腹部开放伤合并生理盐水浸泡组；E组：正常对照组。观察腹腔海水浸泡后肠内容物SIgA及血浆IgA、内毒素（LPS）、肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）含量变化及血、肝脏、肠系膜淋巴结细菌定量培养情况；观察HE染色小肠组织病理损伤评分。结果与E组比较，A组肠内容物SIgA、血浆IgA含量显著下降；血浆LPS、TNF-α、IL-6含量显著升高；发生肠道细菌易位（P〈0.05或P〈0.01）；HE染色显示小肠黏膜组织出现不同程度的损伤（P〈0.05或P〈0.01）。结论腹部开放伤合并海水浸泡后肠道免疫屏障功能显著下降，与内毒素血症和肠道细菌易位的发生密切相关，炎症因子释放及肠黏膜屏障功能损伤是肠道免疫屏障功能受损的重要机制之一。Objective To investigate the changes and meanings of intestinal immune function in rats with open celiac seawater immersion wounds. Methods The rats model of open celiac seawater immersion wounds in rats was built with fifty male Wistar rats which were randomly divided into five groups; celiac seawater immersion group with abdominal open injury （group A） , abdominal open injury group without seawater immersion （ group B） , abdominal seawater immersion group without abdominal open injury （ group C） , celiac physiological saline immersion group with abdominal open injury （group D） and normal control group （group E） to observe the levels of SIgA in intestinal content; IgA, LPS, TNF-α and IL-6 in plasma; BT through quantitative bacterial culture in plasma, liver and MLN in each group, and to observe injury scoring by HE staining intestinal mucosa. Results the levels of SIgA in intestinal content and IgA in plasma decreased significantly in group A. The levels of LPS, TNF-α and IL-6 in plasma increased significantly in group A. The bacterial translocation increased significantly in plasma, liver and MLN in group A（P〈0.05 or P〈0.01）. By HE staining intestinal mucosa, it was observed that different degrees of injuries occurred to the small intestine （P 〈0.05 or P 〈0.01）. Conclusions Celiac seawater immersion can damage intestinal mucosal immune function in rats with open celiac seawater immersion wounds significantly, and it also relates closely with endotoxemia and BT. The release of quantity of cytokine and intestinal mucosal barrier injury are important reasons for intestinal immune function injury.

关 键 词：腹部开放伤 海水浸泡 肠黏膜 免疫屏障 细菌易位 

分 类 号：R686[医药卫生—骨科学]