被动吸烟大鼠椎间盘中白介素-1β和白介素-1受体Ⅰ的表达及意义  

Expression of interleukin-1β,interleukin-1 receptor Ⅰ and their significance in the intervertebral disc of passive cigarett-smoking rats

作　　者：李左安[1] 邵增务[1] 熊小芊[1] 苏彩风[1] 张志才[1] 王河忠[1] 

机构地区：[1]华中科技大学同济医学院协和医院骨科,武汉市430022

出　　处：《中国脊柱脊髓杂志》2008年第7期534-537,I0001,共5页Chinese Journal of Spine and Spinal Cord

摘　　要：目的:观察被动吸烟大鼠椎间盘中白介素-1β(IL-1β)和白介素-1受体Ⅰ(IL-1RⅠ)的表达,探讨被动吸烟诱发椎间盘退变的机制。方法:取4周龄SD大鼠60只,随机分为6组,第1组不予吸烟,10只;第2￣4组分别吸烟2周、4周、8周,第5、6组吸烟8周后予停止吸烟4周、8周,每组10只。在相应时间点处死动物,取L4/5椎间盘行HE染色和IL-1β及IL-1RⅠ免疫组化染色,观察椎间盘退变情况和IL-1β及IL-1RⅠ的表达情况。结果:HE染色显示,吸烟2周时椎间盘无明显退变,吸烟4周时椎间盘出现2￣3级退变,吸烟8周时椎间盘出现3￣4级退变,停止吸烟后4周退变无明显修复;停止吸烟后8周退变部分修复。免疫组化染色显示,吸烟2周组IL-1β及IL-1RⅠ染色阳性细胞率增加,吸烟4周组染色阳性细胞率大于2周组;吸烟8周组达(76±3.2)%和(46±2.8)%,停止吸烟后4周开始下降,8周时降至(66±2.9)%和38±2.2%。结论:被动吸烟可以诱发大鼠椎间盘退变,且随吸烟时间延长退变加重;其导致退变的机制可能与上调椎间盘内IL-1β和IL-1RⅠ的表达有关。Objective ： To observe the expression of interleukin- 1 beta （IL- 1β ）, interleukin- 1 receptor Ⅰ （IL- 1 RⅠ ） in the intervertebral disc of passive cigarette-smoking rats and investigate the possible mechanism of disc degeneration induced by the passive smoking.Method：60 four-week-old rats were used and divided into 6 groups randomly,10 in each group were subjected to passive smoking box to induce intervertebral disc degeneration.Group 1 were used as the control,group 2,3,4 were sacrificed respectively at 2 weeks,4 weeks, 8 weeks of passive smoking,group 5,6 were both given passive smoking for 8 weaks,then group 5 were sacrificed 4 week after cessation of smoking ,group 6 were 8 weeks after cessation of smoking.L4/5 discs were harved,and the histological changes were observed by HE staining,the expression of IL-1β and IL-1 RⅠ was examined by immunohistochemical staining.Result：HE staining showed that there was no obvious disc degeneration in 2 weeks passive smoking group,grade 2 or 3 disc degeneration in 4 weeks group,grade 3 or 4 disc degeneration in 8 weeks group.Cracks,tears,and misalignment of annulus fibrosus were observed clearly in the intervertebral disc 8 weeks after passive smoking,after 8-week cessation of smoking,the disc degeneration could be repaired to some extent.There were more IL-1β,IL-1 RⅠ positive-staining cells 2 weeks after passive smoking,the rate of IL-1β,IL-1 RⅠ posltive-stainlng cells was （76±3.2）% and （46± 2.8）% respectively after 8 weeks of passive smoking.Expression of IL-1β and IL-1 RⅠ decreased at 4 weeks cessation of smoking,the rate of IL-1β,IL-1 RⅠ positive-staining cells were （66±2.9）% and （38± 2.2）% at 8 weeks,both were conspicuously higher than that of non-smoking group （P〈0.01）.Conclusion： Passive smoking could induce irreversible intervertebral disc degeneratlon,with worse degeneration in longer passive smoking.IL-1β ,IL-1 RⅠ may be the important factors in the mechanism of disc degeneration.

关 键 词：椎间盘退变 被动吸烟 白介素-1Β 白介素-1受体Ⅰ 大鼠 

分 类 号：R681.5[医药卫生—骨科学] Q507[医药卫生—外科学]