山羊腰椎间盘损伤后蛋白基因产物9.5免疫组化染色阳性神经纤维的分布  

The expression of protein gene product 9.5(PGP9.5) immunopositive nerve fibers in goat lumbar intervertebral disc after lesion

作　　者：任东风[1] 侯树勋[1] 吴文闻[1] 商卫林[1] 

机构地区：[1]解放军总医院第一附属医院骨科全军骨科研究所,北京市100037

出　　处：《中国脊柱脊髓杂志》2008年第7期538-541,I0002,共5页Chinese Journal of Spine and Spinal Cord

摘　　要：目的:观察山羊腰椎间盘损伤后不同时间点蛋白基因产物9.5(proteingeneproduct9.5,PGP9.5)免疫组化染色阳性神经纤维在损伤椎间盘内的分布,探讨山羊椎间盘损伤后是否出现神经内生长。方法:15只6月龄山羊,用直径1.2mm的穿刺针刀刺伤L5/6椎间盘前纤维环全层和L6/7椎间盘后部纤维环内层,L4/5椎间盘作为对照椎间盘。在造模后3周、3个月、6个月各取5只动物处死,取目标椎间盘光镜下观察椎间盘后部纤维环的组织学改变,检测PGP9.5免疫阳性神经纤维的分布并用半定量方法进行评分。结果:在观察期间L6/7椎间盘后部损伤纤维环未愈合,L4/5椎间盘在各个时间点均未出现裂隙,L5/6椎间盘仅在6个月时有1个椎间盘出现多个裂隙。损伤后3、6个月,在L6/7椎间盘右后1/4区很容易检测到PGP9.5免疫阳性神经纤维,神经纤维沿穿刺道及周围组织向内生长。L4/5和L5/6椎间盘相同区域未检测到相关免疫阳性反应神经纤维。在髓核中没发现PGP9.5免疫染色阳性神经纤维。结论:山羊经椎间盘前方损伤后部纤维环内层后,纤维环内层很难愈合,并出现PGP9.5免疫阳性神经纤维分布,说明椎间盘后部纤维环内层损伤可发生神经内生长。Objective：To study the expression of protein gene product 9.5 （PGP9.5） immunopositive nerve fibers of goat intervertebral disc after lesion at different time interval and explore if the nerve further ingrowth into the goat lumbar intervertebral disc after lesion.Method：15 goats,5 for each group,were subjected to surgery,using puncture knife（1.2mm diameter）,the inner side of posterior annulus fibrosus of L6/7 intervertebral discs were penetrated as the experimental intervertebral disc,the full-thickness of anterior annulus fibrosus of L5/6 intervertebral discs were penetrated as the positive control,L4/5 intervertebral discs as the intact control.The examination of histology from posterior annulus fibrosus of the target discs were taken at the 3 weeks,the 3 and 6 months after operation,the expression of PGP9.5 immunopositive nerve fiber was determined and scored with a semiquantitative method.Result：A total of 15 L6/7 posterior inner annulus fibresus disruption were not healed during experimental session.The fissures of posterior annulus fibrosus were not observed at each time interval in the L4/5 intact control intervertebral disc and the L5/6 intervertebral discs with injured anterior annulus fibrosus.PGP9.5 immunopositive nerve fibers were easily observed in the right posterior outer 1/4 region of L6/7 discs,in addition,the ingrowth of nerve fibers into the disc along the puncture tract and periphery tissue was also observed,the immunopositive nerve fibers were absent in the same regions of L4/5 and L5/6 discs at 3 weeks to 6 months postoperatively.PGP9.5 immunopositive nerve fibers were not observed in the nucleus pulposus of any disc examined.Conclusion：The disruptured inner posterior annulus fibrosus stabbed through anterior lumbar discs of goat is hard to heal,the expression of PGP9.5 immunopositive nerve fiber can be observed in this region.This finding suggests that intervertebral disc injury in inner posterior annulus fibrosus can lead to nerve ingrowth.

关 键 词：椎间盘 纤维环刺伤 神经内生长 免疫组化 

分 类 号：R681.5[医药卫生—骨科学] Q189[医药卫生—外科学]