原癌基因c-src通过磷酸化信号转导与转录激活子-3蛋白影响大鼠精原干细胞活性  

Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3

作　　者：陈加祥[1] 王新长[1] 王晶磊[1] 徐斯凡[2] 秦海燕[1] 杨蓓[1] 杨俊玲[1] 邹挺[1] 

机构地区：[1]南昌大学医学院生理学教研室,江西南昌330006 [2]中央民族大学生命与环境科学学院,北京100081

出　　处：《生理学报》2008年第3期391-396,共6页Acta Physiologica Sinica

基　　金：the National Natural Science Foundation of China (No. 30360032)

摘　　要：本文旨在研究原癌基因c-src在体外培养的9日龄大鼠精原干细胞中的表达及其与精原干细胞活性相关的信号通路。用MTT比色法观察反义c-src脱氧寡核苷酸(oligodeoxynucleotides,ODNs)对精原干细胞作用的量效及时效关系;用RT-PCR检测c-src mRNA的表达;用Western blot检测c-src表达产物pp60c-src和磷酸化的信号转导与转录激活子-3(phosphorylatedsignal transducer and activator of transcription-3,p-STAT3)的表达。结果显示,与空白对照组相比,10μmol/L反义c-srcODNs作用12h后精原干细胞活性下降8.1%(P<0.05),且c-src mRNA表达明显下调;Western blot结果显示反义c-src ODNs组pp60c-src、p-STAT3蛋白表达与空白对照组相比分别下降了33.8%、45.3%(均P<0.01)。以上结果提示,原癌基因c-src可能通过p-STAT3蛋白影响精原干细胞的活性。The present study aimed to investigate the effect of proto-oncogene c-src on the viability of rat spermatogonial stem cells from 9 day-old rat in vitro. MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense c-src oligodeoxynucleotides （ODNs） in vitro; RT-PCR was utilized to observe the expression of c-src mRNA and Western blot was used to observe the protein expressions of pp60c-src and phosphorylated signal transducer and activator of transcription-3 （p-STAT3）. Compared with that in control group, the viability of spermatogonial stem cells decreased by 8.1% （P〈0.05） and the expression of c-src mRNA decreased significantly after treatment with 10μmol/L antisense c-src ODNs for 12 h. Compared with that in the control group, the protein expressions of pp60c-src and p-STAT3 decreased by 33.8% and 45.3% （both P〈0.01）, respectively, in the spermatogonial stem cells after being transfected with antisense c-src ODNs. The results suggest that proto-oncogene c-src regulates the viability of rat spermatogonial stem cells through p-STAT3.

关 键 词：大鼠 精原干细胞 原癌基因c-Src 信号转导与转录激活子-3 

分 类 号：Q344[生物学—遗传学]