黏膜保护剂对实验性胃黏膜损伤及胃黏膜超微结构改变的作用  

Effects of mucosal protective agents against experimental gastric mucosal lesions and ultra-structure changes in gastric mucosal in rats

作　　者：张冬红[1] 董秀云[1] 周丽雅[1] 林三仁[1] 

机构地区：[1]北京大学第三医院消化科100083

出　　处：《中华消化杂志》2008年第5期328-332,共5页Chinese Journal of Digestion

摘　　要：目的评价铝碳酸镁等常用黏膜保护剂对乙醇、阿司匹林、盐酸、泼尼松龙诱发的大鼠胃黏膜损伤的保护作用，并观察铝碳酸镁的黏膜保护作用与胃黏膜上皮细胞间隙变化的关系。方法采用四种方法造模。①乙醇造模：采用雄性Wistar大鼠84只，分为7组，每组12只，分别给予铝碳酸镁、麦滋林、替普瑞酮、吉法酯、硫糖铝、瑞巴派特、0．9％氯化钠溶液3d，第4天动物在给药后再经口给予无水乙醇1ml造模。之后处死动物观察药物对大鼠胃黏膜损伤的作用。计算各组胃黏膜损伤长度（mm）作为损伤指数。②阿司匹林造模：经口给予阿司匹林（300mg／kg）及0.1mol／L盐酸0．5ml／100g造模，余实验方法同①。③盐酸造模：经口给予0．7mol／L盐酸1ml造模，余实验方法同①。④泼尼松龙造模：动物分组及给药剂量同其他模型，给药或0．9％氯化钠溶液5d，第2-5天每天皮下注射泼尼松龙（250mg／kg），第5天处死动物，用Guth法计算损伤指数。取每种动物模型对照组及铝碳酸镁组的第1、5和10号动物胃黏膜组织，用透射电镜检测细胞间隙的变化。结果四种胃黏膜损伤模型中，各黏膜保护剂用药组胃黏膜损伤指数均显著小于对照组（P值均〈0.05），其中铝碳酸镁组与对照组比较差异有统计学意义（P〈0．01）。同时铝碳酸镁组胃黏膜上皮细胞间隙显著小于对照组（P〈0.05）。结论六种常用黏膜保护剂对乙醇、盐酸、阿司匹林、泼尼松龙诱发的胃黏膜损伤均有保护作用，其中铝碳酸镁的保护作用更显著。观察胃黏膜上皮细胞间隙可从细胞学水平进一步证实铝碳酸镁的黏膜保护作用。Objective To evaluate the protective effects of hydrotalcite, Marzulene-s, selbex,gefarnate, sucralfate and rebamipide against the gastrie mucosa lesions induced by ethanol, aspirin, hydrochloric acid or prednisolone in rats. The changes in intercellular space width of gastric epithelial in rats was observed. Methods Four kinds of models were used to observe the protective effects of six agents against the gastric mucosal lesions. ①Ethanol model： a total of 84 male Wistar rats were divided into 7 groups with 12 each. The rats in group 1 to 7 were orally received hydrotalcite, Marzulene-S, gefarnate, sucralfate, rebamipide or normal saline for 3 days, respectively. On day 4, the rats were given 1 ml of absolute ethanol. The length of gastric lesion were measured by ulcer index. 0 Aspirin model： the rats were received 300 mg/kg of aspirin and 0.1 mol/L hydrochloric acid （0. 5 ml/100 g）. The following procedures were as ①.③ Hydrochloric acid model： the rats were received 1 ml of 0.7 mol/L hydrochloric acid. The following procedures were as ①. ④Prednisolone model： all groups were administrated with above 6 agents or normal saline for 5 days. During the 2nd-5th day, the rats were subcutaneously injected with prednisolone （250 mg/kg） daily. Rats were killed on 5th day, and the lesions were measured by ulcer index. Gastric mucosal tissue of No. 1, 5 and 10 rat in the control group and the hydrolalcile group were picked up to measure the intercellular space width using transmission electron microscopy. Results In four kind of models, the ulcer index were significantly lower in ratstreated with mueosal protective agents than that in the controls （P〈0. 05）, expecially in hydrotalcite group （P〈0.01）. The width of intercellular space in the hydrotalcite group was significantly narrower than that in controls （P〈O. 05）. Conclusions All of the mueosal protective agents can be against the gastric mucosal lesion induced by ethanol, hydrochloric acid, aspirin or prednisolone. Among them,

关 键 词：胃黏膜 超微结构 保护剂 铝碳酸镁 

分 类 号：R686[医药卫生—骨科学]