大气污染物对大鼠肺组织CC16及细胞因子mRNA表达的影响  

Effects of Air Pollutants on CC16 and Cytokine mRNA Expression in Pulmonary Tissue of Rats

作　　者：肖纯凌[1] 郭丽[2] 祁荣[2] 席淑华[3] 王任群[3] 

机构地区：[1]沈阳医学院,辽宁沈阳110034 [2]沈阳医学院基础医院中心实验室,辽宁沈阳110034 [3]沈阳医学院公共卫生学院职业医学与环境卫生教研室,辽宁沈阳110034

出　　处：《沈阳医学院学报》2007年第3期129-131,共3页Journal of Shenyang Medical College

基　　金：国家自然科学基金资助项目(No.30471425)

摘　　要：目的：测定大气污染物对大鼠肺组织CC16、TNF-α和IL-6的mRNA表达影响,探讨大气污染物对肺损伤的作用机制。方法：用日本产低流量PM10空气采样器采集PM10颗粒物,采样滤膜用生理盐水超声震荡洗脱,混悬液定容为15mg/ml。48只体重为200-240g Wistar大鼠随机分为3个实验组和1个对照组。实验组大鼠分别气管注入1ml 15mg/ml PM10的生理盐水混悬液,对照组大鼠注入1ml生理盐水。次日,实验组大鼠静态吸入浓度分别为151、2、400mg/m^3的SO2、NO2、CO空气混合气,每天吸入2h,对照组吸入正常空气。于吸入气体污染物1d、7d和30d后次日分别处死实验组和对照组大鼠,取肺组织,采用RT-PCR方法测定TNF-αI、L-6和CC16的mRNA表达水平。结果：吸入大气污染物组在吸入后1d和7d其肺组织CC16mRNA的表达量明显低于对照组;细胞因子TNF-α和IL-6 mRNA表达增强,高于对照组,并于染毒初期即染毒第1d和第7d增高明显,染毒第30d,又呈下降趋势。结论：大气污染物作用于大鼠呼吸系统后,大鼠肺组织CC16 mRNA表达下降,TNF-α和IL-6 mRNA表达增强,并且在污染物作用早期变化明显。Objective： To study mRNA expression of CC16, TNF-α and IL-6 in pulmonary tissue of rats exposed to air pollutants. Methods： Forty-eight Wistar rats were randomly divided into control group and three pollutants exposure groups. Pollutants exposure rats were intratracheal instillated lml PM10 （ 15 mg/ml） physiological saline suspension and control group rats were intratracheal instillated lml physiological saline solution. Next day, three pollutants exposure groups rats were statically inhaled mixed SO2 （15 mg/m^3）, NO2 （12 mg/m^3）, CO （400 mg/m^3） for 2 h per day, and control group rats were given air. The expression in pulmonary tissue of CC16, TNF-α and IL-6 mRNA were examined separately after exposure mixed pollutants 1, 7 and 30 day by RT- PCR. Results： In exposure pollutants groups, CC16 mRNA expression significantly decreased after exposed 1, 7 day, and increased again after 30 days. TNF-α and IL-6 mRNA expression increased in exposure groups after exposed 1, 7 day, and higher than that of control group. At 30 day, TNF-α and IL-6 mRNA expression decreased again. Conclusion： CC16 mRNA expression decrease and TNF-α and IL-6 increase after exposed air pollutants, which change appears early stage of exposure pollutants.

关 键 词：大气污染物 大鼠 CLARA细胞分泌蛋白 TNF-α IL-6 mRNA 

分 类 号：R122.7[医药卫生—环境卫生学]