缺氧诱导因子1α及其靶基因在子痫前期患者胎盘组织中表达的意义  

Study on the Expression of Hypoxia-inducible Factor-1α,VEGF and sFlt-1 in Preeclamptic Placenta

作　　者：尹政[1] 侯丽丽[1] 华政哲[1] 杨秋实[1] 董琳[1] 孙曙光[2] 

机构地区：[1]沈阳医学院 [2]沈阳医学院沈洲医院妇产科

出　　处：《沈阳医学院学报》2007年第3期170-173,共4页Journal of Shenyang Medical College

摘　　要：目的:研究缺氧诱导因子1α(HIF-1α)及其靶基因血管内皮细胞生长因子(VEGF)在子痫前期患者胎盘组织中的表达和相关性,探讨HIF-1α、VEGF在子痫前期发病机制中的作用。方法:采用免疫组化链霉素抗生物素蛋白-过氧化酶连接法(SP)法对20例子痫前期患者(子痫前期组)和15例正常妊娠妇女(对照组)胎盘组织中HIF-1α、VEGF蛋白表达进行半定量分析;应用RT-PCR技术检测两组胎盘组织中HIF-1α、VEGFmRNA的水平并进行相关性分析。结果:(1)免疫组化结果显示,子痫前期组HIF-1α蛋白表达明显增强,其中(+++)阳性表达率分别为45%,显著高于对照组13.3%(P<0.05),而VEGF蛋白表达明显减弱,其中(+++)阳性表达率为15%,显著低于对照组(P<0.01)。(2)子痫前期组HIF-1αmRNA的水平为(0.604±0.013),显著高于对照组的(0.208±0.007),两组比较,差异有统计学意义(P<0.05),VEGF mRNA表达水平虽有升高,但与对照组相比较,差异无统计学意义(P>0.05)。结论:HIF-1α在子痫前期胎盘组织中的表达明显升高,可能通过调节靶基因VEGF的转录影响滋养细胞的浸润功能和胎盘血管重铸,在子痫前期的发病过程中发挥重要作用。Objective： To investigate the expression and correlation of HIF-1α and VEGF in the preeclamptic placenta, aiming to discuss the significance in the pathogenesis of preeclampsia, Methods： Placenta were collected from 20 pregnant women with preeclampsia as study group and 15 normal pregnant women as control group, the expression of HIF-1α and VEGF protein were semi-quantitatively analyzed with immunohistochemicaU assay and mRNA level was determined by using reverse transcription polymerase chain reaction （RT-PCR） technique. Results： （1） The expression of HIF-1α protein in preeclamptic group obviously increased, with （ ＋ ＋ ＋ ） positive expression rate signifi- cantly higher than control group （ P 〈 0.05）, but VEGF expression obviously reduced in preeclamptic group （ P 〈 0.01 ）. （2） The level of HIF-1α mRNA in preeclamptic placenta was significantly higher than normal placenta （ P 〈 0.05）, although the level of VEGF mRNA increased in preeclamptic placenta, but not significantly different from normal placenta （ P 〉 0.05）. Conclusion： The abnormal higher HIF-1α expression in preeclamptic placenta indicated that HIF-1α might play an important role in the pathogenesis of preeclampsia, possibly through affecting the cytotrophblastic invasion and placental vascular reconstruction via the modulation of VEGF gene transcription.

关 键 词：妊娠并发症 缺氧诱导因子1Α 血管内皮细胞生长因子 受体 

分 类 号：R714.24[医药卫生—妇产科学]